Anti-beta 2 Microglobulin antibody (ab223571)
Key features and details
- Goat polyclonal to beta 2 Microglobulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta 2 Microglobulin antibody
See all beta 2 Microglobulin primary antibodies -
Description
Goat polyclonal to beta 2 Microglobulin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human beta 2 Microglobulin aa 1-100 (N terminal) (Cysteine residue). (NP_004039.1).
Database link: P61769 -
Positive control
- WB: Human duodenum lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab223571 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 14 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Database links
- Entrez Gene: 450170 Chimpanzee
- Entrez Gene: 280729 Cow
- Entrez Gene: 101867173 Cynomolgus monkey
- Entrez Gene: 101132159 Gorilla
- Entrez Gene: 567 Human
- Entrez Gene: 712428 Rhesus monkey
- Omim: 109700 Human
- SwissProt: P61770 Chimpanzee
see all -
Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
Images
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Anti-beta 2 Microglobulin antibody (ab223571) at 0.3 µg/ml + human duodenum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 14 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab223571 has not yet been referenced specifically in any publications.