Anti-beta 2 Adrenergic Receptor antibody (ab107217)

Overview

  • Product nameAnti-beta 2 Adrenergic Receptor antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Chicken polyclonal to beta 2 Adrenergic Receptor
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide derived from within residues 1 - 100 of Human beta 2 Adrenergic Receptor.

    (Peptide available as ab38102.)

  • Positive control
    • This antibody gave a positive signal in Human, Mouse and Rat Brain Tissue Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab107217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor antibody images

  • All lanes : Anti-beta 2 Adrenergic Receptor antibody (ab107217) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-beta 2 Adrenergic Receptor antibody (ab107217)

ab107217 has not yet been referenced specifically in any publications.

Product Wall


I am very pleased to hear you would like to accept our offer and test ab107217 in ICC/IF. This code will give you €370 off your next order before the expiration date above. To redeem this offer, please submit an Abreview for ICC/IF and includ...

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To our knowledge, ab107217 has not been tested in ICC-IF. However by participating in our AbTrial program you can now use o...

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I would be happy to raise the testing discount for you, however, in order to do this I would need to know which application you are hoping to use the antibody in. The antibody would be guaranteed with your mouse sampl...

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Thank you for contacting us and your interest in our products.

The Anti-beta 2 Adrenergic Receptor antibody (ab13989) has been shown to recognize the human and rat from of the protein, however, we have as yet not tested the antibody with mous...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"