Anti-beta 2 Adrenergic Receptor antibody (ab13989)

Overview

  • Product nameAnti-beta 2 Adrenergic Receptor antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Chicken polyclonal to beta 2 Adrenergic Receptor
  • Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    DFRIAFQELLC LRRSSLKAYGN GYSSNGNTGE QSGYHVEQEKE NKLLCEDLPGT EDFVGHQGTVP SDNIDSQGRNCS T

    , corresponding to amino acids 335-408 of Human beta 2 Adrenergic Receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab13989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
ICC 1/1000.
WB 1/500. Predicted molecular weight: 46.5 kDa.

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor antibody images



  • Predicted band size : 46.5 kDa


    E coli-derived fusion protein as test antigen. Affi-pure IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. Colorimetric method for signal development.

References for Anti-beta 2 Adrenergic Receptor antibody (ab13989)

This product has been referenced in:
  • Valentine CD & Haggie PM Confinement of ß(1)- and ß(2)-adrenergic receptors in the plasma membrane of cardiomyocyte-like H9c2 cells is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins but not caveolae. Mol Biol Cell 22:2970-82 (2011). Read more (PubMed: 21680711) »
  • Koryakina YA  et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF ; Human, Rat . Read more (PubMed: 18423037) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (astrocytes)
Permeabilization Yes - TX100 0.05%
Specification astrocytes
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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Submitted Jul 28 2016

Application Western blot
Sample Rat Cell lysate - whole cell (Astrocyte culture)
Gel Running Conditions Reduced Denaturing (gel 10%)
Loading amount 1.7 µg
Specification Astrocyte culture
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 2°C
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Submitted Sep 29 2015

Thank you very much for your reply which has been forwarded to me as my colleague #### is away from the office today.

To our knowledge, ab107217 has not been tested in ICC-IF. However by participating in our AbTrial program you can now use o...

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Thank you for contacting us and your interest in our products.

The Anti-beta 2 Adrenergic Receptor antibody (ab13989) has been shown to recognize the human and rat from of the protein, however, we have as yet not tested the antibody with mous...

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Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody.  Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if...

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I have received word from the laboratory that the following cell lines should serve as a good positive control: HEL K-562 NB-4 U-937 Karpas-707 KM3 LP-1 U-698 D341 Med ...

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Thank you for your reply and for providing the requested information. After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (HEK 293 cells stably expressing human beta2-AR)
Specification HEK 293 cells stably expressing human beta2-AR
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 Triton X -100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted May 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (HEK 293 cells transiently expressing rat beta2-AR)
Specification HEK 293 cells transiently expressing rat beta2-AR
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
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Submitted May 09 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary Cultures of Rat Airway Smooth Muscle and E)
Specification Primary Cultures of Rat Airway Smooth Muscle and E
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
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Submitted May 07 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"