The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 47,65 kDa.
Use at an assay dependent concentration.
(Cellular and membrane)
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation. Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor. Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
Cellular ELISA: HEK293 cells expressing beta 2 Adrenergic Receptor were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
Membrane ELISA: Lewis rat brain membranes (5 µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with ab176490 (1/1000 of a stock solution of 1µg/μL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).