Anti-beta 2 Adrenergic Receptor (phospho S346) antibody (ab62464)

Overview

  • Product nameAnti-beta 2 Adrenergic Receptor (phospho S346) antibody
    See all beta 2 Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to beta 2 Adrenergic Receptor (phospho S346)
  • Specificityab62464 detects endogenous levels of beta 2 Adrenergic Receptor only when phosphorylated at serine 346.
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human beta 2 Adrenergic Receptor around the phosphorylation site of serine 346.

  • Positive control
    • Human brain tissue and Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
  • Post-translational
    modifications
    Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
    Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
    Ubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • ADRB2_HUMAN antibody
    • ADRB2R antibody
    • ADRBR antibody
    • Adrenergic beta 2 receptor surface antibody
    • Adrenoceptor beta 2 surface antibody
    • B2AR antibody
    • BAR antibody
    • beta 2 adrenoceptor antibody
    • Beta 2 adrenoreceptor antibody
    • Beta-2 adrenergic receptor antibody
    • Beta-2 adrenoceptor antibody
    • Beta-2 adrenoreceptor antibody
    • BETA2AR antibody
    • Catecholamine receptor antibody
    • OTTHUMP00000160386 antibody
    see all

Anti-beta 2 Adrenergic Receptor (phospho S346) antibody images

  • ab62464, at 1/50 dilution, staining beta 2 Adrenergic Receptor in paraffin-embedded human brain tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
  • ab62464, at 1/500 dilution, staining beta 2 Adrenergic Receptor in Jurkat cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References for Anti-beta 2 Adrenergic Receptor (phospho S346) antibody (ab62464)

ab62464 has not yet been referenced specifically in any publications.

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