Anti-beta 2 Defensin/BD-2 antibody (ab63982)
Key features and details
- Rabbit polyclonal to beta 2 Defensin/BD-2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-beta 2 Defensin/BD-2 antibody
See all beta 2 Defensin/BD-2 primary antibodies -
Description
Rabbit polyclonal to beta 2 Defensin/BD-2 -
Host species
Rabbit -
Specificity
Human 7 kDa beta 2 Defensin / hBD-2.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Has antibacterial activity. -
Tissue specificity
Expressed in the skin and respiratory tract. -
Sequence similarities
Belongs to the beta-defensin family. LAP/TAP subfamily. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100289462 Human
- Entrez Gene: 1673 Human
- Omim: 602215 Human
- SwissProt: O15263 Human
- Unigene: 105924 Human
- Unigene: 721744 Human
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Alternative names
- BD 2 antibody
- BD-2 antibody
- BD2 antibody
see all
Images
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ab63982 (1/500) staining beta 2 Defensin/BD-2 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of a small population of cells within the tonsillar tissue.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
ab63982 staining beta 2 Defensin/BD-2 in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/500 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab63982 has been referenced in 20 publications.
- Dajnoki Z et al. Primary alterations during the development of hidradenitis suppurativa. J Eur Acad Dermatol Venereol 36:462-471 (2022). PubMed: 34724272
- Lebeau A et al. HPV infection alters vaginal microbiome through down-regulating host mucosal innate peptides used by Lactobacilli as amino acid sources. Nat Commun 13:1076 (2022). PubMed: 35228537
- Šerstņova K et al. Expression of anti-inflammatory markers IL-2, IL-10, TGF-β1, βDEF-2, βDEF-3 and Cathelicidin LL37 in dairy cattle milk with different health status of the udder. Pol J Vet Sci 25:237-248 (2022). PubMed: 35861957
- Meloni M et al. Reproducing the scalp microbiota community: co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta. Int J Cosmet Sci 43:235-245 (2021). PubMed: 33469935
- Woodby B et al. Cutaneous antimicrobial peptides: New "actors" in pollution related inflammatory conditions. Redox Biol 41:101952 (2021). PubMed: 33839421