Overview

  • Product nameAnti-beta 2 Defensin antibody
    See all beta 2 Defensin primary antibodies
  • Description
    Rabbit polyclonal to beta 2 Defensin
  • SpecificityHuman 7 kDa beta 2 Defensin.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from beta 2 Defensin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 7 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionHas antibacterial activity.
  • Tissue specificityExpressed in the skin and respiratory tract.
  • Sequence similaritiesBelongs to the beta-defensin family. LAP/TAP subfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 2 antibody
    • BD-2 antibody
    • BD2 antibody
    • beta 2 antibody
    • Beta defensin 2 antibody
    • Beta defensin 4B antibody
    • Beta-defensin 2 antibody
    • Beta-defensin 4A antibody
    • DEF B2 antibody
    • DEF B4 antibody
    • DEFB 102 antibody
    • DEFB 2 antibody
    • DEFB 4 antibody
    • DEFB102 antibody
    • DEFB2 antibody
    • DEFB2, formerly antibody
    • DEFB4 antibody
    • DEFB4, formerly antibody
    • DEFB4B antibody
    • DEFB4P antibody
    • Defensin antibody
    • Defensin beta 2 antibody
    • Defensin, beta 4 antibody
    • Defensin, beta 4, pseudogene antibody
    • Defensin, beta 4A antibody
    • Defensin, beta 4B antibody
    • Defensin, beta, 2, formerly antibody
    • Defensin, beta, 4, formerly antibody
    • DFB4A_HUMAN antibody
    • HBD 2 antibody
    • hBD-2 antibody
    • HBD2 antibody
    • mBD-2 antibody
    • SAP 1 antibody
    • SAP1 antibody
    • Skin antimicrobial peptide 1 antibody
    • Skin-antimicrobial peptide 1 antibody
    see all

Anti-beta 2 Defensin antibody images

  • ab63982 (1/500) staining beta 2 defensin in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of a small population of cells within the tonsillar tissue.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ab63982 staining beta 2 Defensin in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/500 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References for Anti-beta 2 Defensin antibody (ab63982)

This product has been referenced in:
  • Qian YJ  et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human ß Defensins Expression in Human Oral Mucosa. Cell Physiol Biochem 36:457-73 (2015). Human . Read more (PubMed: 25968832) »
  • Introini A  et al. Expression profiles of antimicrobial peptides in the genital tract of women using progesterone intrauterine devices versus combined oral contraceptives. Am J Reprod Immunol 72:475-84 (2014). IHC (PFA fixed) ; Human . Read more (PubMed: 25087845) »

See all 5 Publications for this product

Product Wall

The exact immunogen sequence for these antibodies is proprietary so I am sorry we are unable to disclose this information. The immunogen however corresponds to the mature form of Beta 2 defensin protein. We do have sequence listed for ab14426 and ab66...

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Thank you for your enquiry.

I am sorry to confirm that ab63982beta 2 Defensin antibodyhas not yet been tested in ICC-If. It has been tested in human samples.I am pleased to hear the customer is interested in using our testing discount progra...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreatic)
Specification Pancreatic
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9
Permeabilization No
Username

Dr. Ilka Werner-Martini

Verified customer

Submitted Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"