Anti-beta 2 Defensin antibody (ab66072)

Overview

  • Product nameAnti-beta 2 Defensin antibody
    See all beta 2 Defensin primary antibodies
  • Description
    Mouse monoclonal to beta 2 Defensin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment with tag: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P, corresponding to amino acids 24-65 of Human beta 2 Defensin

  • Positive control
    • Beta 2 Defensin transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged beta 2 Defensin is approximately 3ng/ml as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).

Target

  • FunctionHas antibacterial activity.
  • Tissue specificityExpressed in the skin and respiratory tract.
  • Sequence similaritiesBelongs to the beta-defensin family. LAP/TAP subfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BD 2 antibody
    • BD-2 antibody
    • BD2 antibody
    • beta 2 antibody
    • Beta defensin 2 antibody
    • Beta defensin 4B antibody
    • Beta-defensin 2 antibody
    • Beta-defensin 4A antibody
    • DEF B2 antibody
    • DEF B4 antibody
    • DEFB 102 antibody
    • DEFB 2 antibody
    • DEFB 4 antibody
    • DEFB102 antibody
    • DEFB2 antibody
    • DEFB2, formerly antibody
    • DEFB4 antibody
    • DEFB4, formerly antibody
    • DEFB4B antibody
    • DEFB4P antibody
    • Defensin antibody
    • Defensin beta 2 antibody
    • Defensin, beta 4 antibody
    • Defensin, beta 4, pseudogene antibody
    • Defensin, beta 4A antibody
    • Defensin, beta 4B antibody
    • Defensin, beta, 2, formerly antibody
    • Defensin, beta, 4, formerly antibody
    • DFB4A_HUMAN antibody
    • HBD 2 antibody
    • hBD-2 antibody
    • HBD2 antibody
    • mBD-2 antibody
    • SAP 1 antibody
    • SAP1 antibody
    • Skin antimicrobial peptide 1 antibody
    • Skin-antimicrobial peptide 1 antibody
    see all

Anti-beta 2 Defensin antibody images

  • All lanes : Anti-beta 2 Defensin antibody (ab66072) at 1 µg/ml

    Lane 1 : beta 2 Defensin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 8 kDa
    Observed band size : 8 kDa

References for Anti-beta 2 Defensin antibody (ab66072)

ab66072 has not yet been referenced specifically in any publications.

Product Wall

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

Here are my thoughts:

1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

Read More

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"