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Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)

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Overview

Product name

Anti-beta 2 Microglobulin antibody [B2M-01]
See all beta 2 Microglobulin products (28) ...

Description

Mouse monoclonal [B2M-01] to beta 2 Microglobulin

Specificity

This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.

Tested applications

ELISA, Flow Cyt, IP, RIA, WBmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rabbit, Chicken, Cow, Dog, Pig

Immunogen

Full length native protein (purified) (Human).

Positive control

U-937 and RAJI cell lysates

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Purified from ascites using precipitation methods and DEAE-chromatography.

Clonality

Monoclonal

Clone number

B2M-01

Isotype

IgG2a

Research areas

Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - beta 2 Microglobulin antibody [B2M-01] (ab759)Western blot - beta 2 Microglobulin antibody [B2M-01] (ab759) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ELISA

     ELISA: Use at an assay d...Read more →

    ELISA: Use at an assay dependent dilution.

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    Flow Cyt

     Flow Cyt: Use at an assa...Read more →

    Flow Cyt: Use at an assay dependent dilution.

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    RIA

      See more...Read more →

    RIA: Use at an assay dependent dilution. (Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.)

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2 µg/ml Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).(Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs. )

Target

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Involvement in disease

Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

Sequence similarities

Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Post-translational
modifications

Glycation of Ile-21 is observed in long-term hemodialysis patients.

Cellular localization

Secreted. Detected in serum and urine.

Target information above from: UniProt accessionP61769 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B2M antibody
  • B2MG_HUMAN antibody
  • Beta 2 microglobulin precursor antibody
  • Beta chain of mhc class 1 proteins antibody
  • Beta-2-microglobulin form pI 5.3 antibody
  • Hdcma22p antibody
see all

Database links

Anti-beta 2 Microglobulin antibody [B2M-01] images:

  Western blot - beta 2 Microglobulin antibody [B2M-01] (ab759)

Western blot - beta 2 Microglobulin antibody [B2M-01] (ab759)

All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

Lane 1 : JEG-3 cell lysate
Lane 2 : A-431 cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : Raji cell lysate

developed using the ECL technique

Performed under non-reducing conditions.

Predicted band size : 13 kDa


Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.

References for Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)

This product has been referenced in:

  • Tiwari Set al. Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J Cell Sci 124:1672-80 (2011).Read more (PubMed: 21511727) »
  • Slany Aet al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB; Human.Read more (PubMed: 19678649) »

See all 8 publications for this product

Publishing research using ab759? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"