beta 2 Microglobulin antibody [B2M-01] (ab759)

Product Name 

beta 2 Microglobulin antibody [B2M-01]

Product type 

Primary antibodies

Description 

Mouse monoclonal [B2M-01] to beta 2 Microglobulin

Immunogen 

Full length native protein (purified) (Human).

Reacts with 

Hu

Does not react with 
(species key)

Ms, Chk, Cow, Dog, Pig, Rb

Specificity 

This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.

Tested applications 
(see key)

ELISA, Flow Cyt, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependant concentration.
Flow Cyt: Use at an assay dependant concentration.
IP: Use at an assay dependant concentration.
RIA: Use at an assay dependant concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIA
WB: Use at a concentration of 2 µg/ml. Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

U-937 and RAJI cell lysates

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Blood >> Blood Cell Antigens >> WBC Antigens >> HLA
Cancer >> Tumor biomarkers >> Globulins
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Beta 2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

B2M-01

Isotype 

IgG2a

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from ascites using precipitation methods and DEAE-chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for beta 2 Microglobulin antibody [B2M-01] (ab759)

  This product has been used in: (two most recent references)

Larrea E et al. Oncostatin M enhances the antiviral effects of type I interferon and activates immunostimulatory functions in liver epithelial cells. J Virol 83:3298-311 (2009). WB; Human. PubMed: 19158240

Kanda Y et al. Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types. Glycobiology 17:104-18 (2007). PubMed: 17012310

See all 6 publication references for this product.


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