Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)


  • Product nameAnti-beta 2 Microglobulin antibody [B2M-01]
    See all beta 2 Microglobulin primary antibodies
  • Description
    Mouse monoclonal [B2M-01] to beta 2 Microglobulin
  • SpecificityThis antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.
  • Tested applicationsSuitable for: ELISA, Flow Cyt, IP, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog, Pig
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • U-937 and RAJI cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites using precipitation methods and DEAE-chromatography.
  • ClonalityMonoclonal
  • Clone numberB2M-01
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA.
WB Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.


  • FunctionComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in diseaseDefects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similaritiesBelongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localizationSecreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    see all

Anti-beta 2 Microglobulin antibody [B2M-01] images

  • All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

    Lane 1 : JEG-3 cell lysate
    Lane 2 : A-431 cell lysate
    Lane 3 : U-937 cell lysate
    Lane 4 : Raji cell lysate

    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 13 kDa

References for Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)

This product has been referenced in:
  • Tiwari S  et al. Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J Cell Sci 124:1672-80 (2011). Read more (PubMed: 21511727) »
  • Slany A  et al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB ; Human . Read more (PubMed: 19678649) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.1M PBS with 3% TritonX100
Fixative Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 14 2014

Thank you for contacting us for technical support regarding ab759. I have contacted the laboratory and was able to find out that indeed non reducing conditions are advised with this antibody. I have added immediately on our datasheet (accessible belo...

Read More

Thank you for your enquiry. This antibody does not cross react with MHC class I heavy chain.

We do not have any problems with this clone. We have 3 units that are in stock currently.