Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
Key features and details
- Mouse monoclonal [B2M-01] to beta 2 Microglobulin
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-beta 2 Microglobulin antibody [B2M-01]
See all beta 2 Microglobulin primary antibodies -
Description
Mouse monoclonal [B2M-01] to beta 2 Microglobulin -
Host species
Mouse -
Specificity
This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA. -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rabbit, Chicken, Cow, Dog, Pig -
Immunogen
Full length native protein (purified) (Human).
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Positive control
- U-937 and RAJI cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified by precipitation methods. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
B2M-01 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs. |
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Flow Cyt |
Use a concentration of 0.5 - 4 µg/ml.
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Notes |
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WB
Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs. |
Flow Cyt
Use a concentration of 0.5 - 4 µg/ml. |
Target
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
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Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
Images
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All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml
Lane 1 : Raji cell lysate under non-reducing condition
Lane 2 : Jurkat cell lysate under non-reducing condition
Lane 3 : HeLa cell lysate under non-reducing condition
Lane 4 : Daudi cell lysate under non-reducing condition
Lane 5 : Raji cell lysate under reducing condition
Lane 6 : Jurkat cell lysate under reducing condition
Lane 7 : HeLa cell lysate under reducing condition
Lane 8 : Daudi cell lysate under reducing condition
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody
Predicted band size: 13 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted? -
Flow cytometric analysis of Human Peripheral Blood cells labelling beta 2 Microglobulin with ab759 at 0.3 ug/ml showing separation of human monocytes (red-filled) from human beta2-Microglobulin negative blood debris (black-dashed).
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All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml
Lane 1 : JEG-3 cell lysate
Lane 2 : A-431 cell lysate
Lane 3 : U-937 cell lysate
Lane 4 : Raji cell lysate
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 13 kDa
Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab759 has been referenced in 11 publications.
- Rodríguez-Rodríguez M et al. Activation of Peptidylarginine Deiminase in the Salivary Glands ofBalb/cMice Drives the Citrullination of Ro and La Ribonucleoproteins. J Immunol Res 2017:8959687 (2017). PubMed: 29318161
- Raha-Chowdhury R et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). WB . PubMed: 25896789
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467
- Tiwari S et al. Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J Cell Sci 124:1672-80 (2011). PubMed: 21511727
- Slany A et al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB ; Human . PubMed: 19678649