Anti-beta 2 Microglobulin antibody [B2M-01] (Phycoerythrin) (ab49424)

Overview

  • Product name
    Anti-beta 2 Microglobulin antibody [B2M-01] (Phycoerythrin)
    See all beta 2 Microglobulin primary antibodies
  • Description
    Mouse monoclonal [B2M-01] to beta 2 Microglobulin (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    This antibody reacts with beta 2 microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin. Beta 2 mircoglobulin is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog
  • Immunogen

    Full length native protein (purified) (Human)

  • Positive control
    • Peripheral blood lymphocytes.
  • General notes

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Properties

Applications

Our Abpromise guarantee covers the use of ab49424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml.

Target

  • Function
    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • Involvement in disease
    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • Sequence similarities
    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • Cellular localization
    Secreted. Detected in serum and urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    • IMD43 antibody
    see all

References

This product has been referenced in:
  • Khurana M  et al. Urine proteomic profiling of pediatric nephrotic syndrome. Pediatr Nephrol 21:1257-65 (2006). Read more (PubMed: 16810512) »
  • Hilgert I  et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984). Read more (PubMed: 6394369) »

See all 2 Publications for this product

Customer reviews and Q&As

Vielen Dank für die Anfrage. Dass ab46793 nicht auf der Liste auftaucht, bedeutet dass er nicht getestet wurde, ob er mit Maus kreuzreagiert. In der Tat ist ab46793 für die Immunhistochemie (IHC-P/Fr - neben anderen Anwendungen wie Durchflusszy...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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