Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01] (ab187602)
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [B2M01] to beta 2 Microglobulin
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 647 Anti-beta 2 Microglobulin antibody [B2M01]
See all beta 2 Microglobulin primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [B2M01] to beta 2 Microglobulin -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Specificity
ab187602 reacts with beta 2 microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 microglobulin. -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rabbit, Chicken, Cow, Dog -
Immunogen
Full length native protein (purified) corresponding to Human beta 2 Microglobulin.
Database link: P61769 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
B2M01 -
Isotype
IgG2a -
Research areas
Associated products
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
Sequence similarities
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Post-translational
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients. -
Cellular localization
Secreted. Detected in serum and urine. - Information by UniProt
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Database links
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
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Alternative names
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence of Human beta 2 Microglobulin-transfected canine cells labeled A) with ab187602 and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml.
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Flow Cytometry analysis of lymphocytes stained using ab187602 (red) at 5µg/ml, separated from unstained lymphocytes (black-dashed).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab187602 has not yet been referenced specifically in any publications.