The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. ab176104-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in disease
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Belongs to the beta-2-microglobulin family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Immunocytochemistry/ Immunofluorescence of Human beta 2 Microglobulin-transfected canine cells labeled A) with ab187602 and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml.