The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesAP: Use at an assay dependent dilution.
Flow Cyt: Use 1µg for 106 cells.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 13 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in diseaseDefects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation. Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similaritiesBelongs to the beta-2-microglobulin family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Post-translational modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localizationSecreted. Detected in serum and urine.