Anti-beta 3 Adrenergic Receptor antibody (ab59685)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab59685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 23283549ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

Target

  • FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
  • Tissue specificityExpressed mainly in adipose tissues.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADR B3 antibody
    • ADRB 3 antibody
    • ADRB3 antibody
    • ADRB3_HUMAN antibody
    • ADRB3R antibody
    • Adrenergic beta 3 receptor antibody
    • B3AR antibody
    • Beta 3 adrenoceptor antibody
    • Beta 3 adrenoreceptor antibody
    • Beta 3AR antibody
    • Beta-3 adrenergic receptor antibody
    • Beta-3 adrenoceptor antibody
    • Beta-3 adrenoreceptor antibody
    • Beta3AR antibody
    • FLJ99960 antibody
    see all

Anti-beta 3 Adrenergic Receptor antibody images

  • ICC/IF image of ab59685 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59685, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-beta 3 Adrenergic Receptor antibody (ab59685)

This product has been referenced in:
  • Okumura T  et al. Phospholipase C-Related Catalytically Inactive Protein (PRIP) Regulates Lipolysis in Adipose Tissue by Modulating the Phosphorylation of Hormone-Sensitive Lipase. PLoS One 9:e100559 (2014). WB ; Mouse . Read more (PubMed: 24945349) »
  • Salisbury EA  et al. Transient brown adipocyte-like cells derive from peripheral nerve progenitors in response to bone morphogenetic protein 2. Stem Cells Transl Med 1:874-85 (2012). Flow Cyt ; Mouse . Read more (PubMed: 23283549) »

See all 2 Publications for this product

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The antibodies ab101095 and ab59685 are fully characterized with rat sample and both are guaranteed for western blot. The differences between the two is in concentration, host species, storage buffer and immunogen ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (white adipose tissue)
Loading amount 50 µg
Specification white adipose tissue
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"