The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBeta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
Tissue specificityExpressed mainly in adipose tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
Western blot - beta 3 Adrenergic Receptor antibody (ab77588)
Anti-beta 3 Adrenergic Receptor antibody (ab77588) at 0.03 µg/ml + human brain (cerebral cortex) lysate (in RIPA buffer) at 35 µg
Predicted band size : 45 kDa Observed band size : 45 kDa
References for Anti-beta 3 Adrenergic Receptor antibody (ab77588)
This product has been referenced in:
O'Leary AP et al. Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration Via a cAMP-Dependent Mechanism and Wound Angiogenesis. J Cell PhysiolN/A:N/A (2014).
Read more (PubMed: 24986762) »