![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 4 Defensin antibody [L13-10-D1] (ab14419) image](#beta-4-Defensin-Primary-antibodies-ab14419-3.jpg)
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Products:Microbiology >> Protein >> Human Protein >> Defensin
Overview
Product name
Anti-beta 4 Defensin antibody [L13-10-D1]
See all beta 4 Defensin products (4) ...
Description
Mouse monoclonal [L13-10-D1] to beta 4 Defensin
Tested applications
ELISA, WB, RIA, IHC-P, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: ELDRICGYGT ARCRKKCRSQ EYRIGRCPNT YACCLRK), corresponding to amino acids 25-61 of Human beta 4 Defensin (precursor, SwissProt Q8WTQ1).
ELDRICGYGT ARCRKKCRSQ EYRIGRCPNT YACCLRK)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50mM Tris HCl. pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
L13-10-D1
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Microbiology >> Protein >> Human Protein >> Defensin
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 4 Defensin antibody [L13-10-D1] (ab14419)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 4 Defensin antibody [L13-10-D1] (ab14419) image](/ps/datasheet/images/14/ab14419/beta-4-Defensin-Primary-antibodies-ab14419-3.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
RIA
- 1 Image
IHC-P
Application notes
ELISA: Use at an assay dependent dilution.
IHC-P: Tested in uterus and kidney sections. Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 8 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
Tissue specificity
High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
Sequence similarities
Belongs to the beta-defensin family.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ8WTQ1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- beta 104 antibody
- BD 4 antibody
- BD-4 antibody
see all
Database links
- Entrez Gene: 140596 Human
- Entrez Gene: 503618 Human
- Omim: 602215 Human
- SwissProt: Q8WTQ1 Human
- Unigene: 559507 Human
- Unigene: 730153 Human
Anti-beta 4 Defensin antibody [L13-10-D1] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta 4 Defensin antibody [L13-10-D1] (ab14419)
ab14419 staining beta 4 Defensin in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/100 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat IgG polyclonal was used as the secondary antibody.
Image courtesy of Dr Ilka Werner-Martini by Abreview
References for Anti-beta 4 Defensin antibody [L13-10-D1] (ab14419)
This product has been referenced in:
- Dréno Bet al. Hidradenitis Suppurativa: The Role of Deficient Cutaneous Innate Immunity. Arch Dermatol : (2011). ICC/IF; Human.Read more (PubMed: 22004878) »
- Garreis Fet al. Roles of human beta-defensins in innate immune defense at the ocular surface: arming and alarming corneal and conjunctival epithelial cells. Histochem Cell Biol 134:59-73 (2010). IHC-P; Human, Mouse.Read more (PubMed: 20526610) »
See all 2 publications for this product
Publishing research using ab14419? Please let us know so that we can cite the reference in this datasheet
![beta 4 Defensin antibody [L13-10-D1] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (14419)](/ps/datasheet/images/14/ab14419/beta-4-Defensin-Primary-antibodies-ab14419-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"