Anti-beta 4 Defensin antibody [L13-10-D1] (ab14419)

Overview

  • Product name
    Anti-beta 4 Defensin antibody [L13-10-D1]
    See all beta 4 Defensin primary antibodies
  • Description
    Mouse monoclonal [L13-10-D1] to beta 4 Defensin
  • Tested applications
    Suitable for: ELISA, WB, RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: ELDRICGYGT ARCRKKCRSQ EYRIGRCPNT YACCLRK), corresponding to amino acids 25-61 of Human beta 4 Defensin (precursor, SwissProt Q8WTQ1).

Properties

Applications

Our Abpromise guarantee covers the use of ab14419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
RIA
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: Tested in uterus and kidney sections. Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 8 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103.
    • Tissue specificity
      High expression in the testis. Gastric antrum exhibited relatively high levels. A lower expression is observed in uterus and neutrophils thyroid gland, lung, and kidney. No detectable expression in other tissues tested.
    • Sequence similarities
      Belongs to the beta-defensin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • BD 4 antibody
      • BD-4 antibody
      • BD4 antibody
      • beta 104 antibody
      • Beta defensin 4 antibody
      • Beta-defensin 104 antibody
      • Beta-defensin 4 antibody
      • D104A_HUMAN antibody
      • DEFB-4 antibody
      • DEFB104B antibody
      • Defensin antibody
      • Defensin beta 104 antibody
      • Defensin beta 4 precursor antibody
      • hBD 4 antibody
      • hBD-4 antibody
      • hBD4 antibody
      see all

    Anti-beta 4 Defensin antibody [L13-10-D1] images

    • ab14419 staining beta 4 Defensin in human pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then incubated with the primary antibody at a 1/100 dilution for 1 hour at 25°C. An undiluted HRP-conjugated goat IgG polyclonal was used as the secondary antibody.

      See Abreview

    References for Anti-beta 4 Defensin antibody [L13-10-D1] (ab14419)

    This product has been referenced in:
    • Dréno B  et al. Hidradenitis Suppurativa: The Role of Deficient Cutaneous Innate Immunity. Arch Dermatol : (2011). ICC/IF ; Human . Read more (PubMed: 22004878) »
    • Garreis F  et al. Roles of human beta-defensins in innate immune defense at the ocular surface: arming and alarming corneal and conjunctival epithelial cells. Histochem Cell Biol 134:59-73 (2010). IHC-P ; Human, Mouse . Read more (PubMed: 20526610) »

    See all 2 Publications for this product

    Product Wall

    Has our customer had a chance to test the protein concentrations via Bradford assay?

    I have unfortunately been unable to locate western blot data. Based on the information it may be that a native western blot system is required. It is also po...

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    Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

    Here are my thoughts:

    1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreas)
    Specification
    Pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer pH9
    Permeabilization
    No
    Username

    Dr. Ilka Werner-Martini

    Verified customer

    Submitted Oct 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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