The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Western blot - beta Actin antibody [1E7] (ab93027)
All lanes : Anti-beta Actin antibody [1E7] (ab93027) at 5 µg/ml
Lane 1 : recombinant protein (immunogen) at 0.2 µg Lane 2 : PC 12 cell lysate at 50 µg Lane 3 : Raw 264.7 cell lysate at 50 µg Lane 4 : Jurkat cell lysate at 50 µg Lane 5 : NIH/3T3 cell lysate at 50 µg
ICC/IF image of ab93027 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93027, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-beta Actin antibody [1E7] (ab93027)
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