Anti-beta Actin antibody [1E7] (ab93027)

Overview

• Product nameAnti-beta Actin antibody [1E7]See all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [1E7] to beta Actin
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant full length Human beta Actin protein (AAH01301) with a proprietary 26 kDa tag.

• Positive controlHuman stomach tissue; NIH/3T3, Raw 264.7, Jurkat and PC 12 cell lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone number1E7
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P60711 Rat
  • Unigene: 520640 Human

  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A26C1A antibodyA26C1B antibodyACTB antibody

  ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody [1E7] images

• 

  Western blot - beta Actin antibody [1E7] (ab93027)
  All lanes : Anti-beta Actin antibody [1E7] (ab93027) at 5 µg/ml
  
  Lane 1 : recombinant protein (immunogen) at 0.2 µg
  Lane 2 : PC 12 cell lysate at 50 µg
  Lane 3 : Raw 264.7 cell lysate at 50 µg
  Lane 4 : Jurkat cell lysate at 50 µg
  Lane 5 : NIH/3T3 cell lysate at 50 µg
  
  
  Predicted band size : 42 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Actin antibody [1E7] (ab93027)
  ab93027, at 3 µg/ml, staining beta Actin in formalin fixed, paraffin embedded Human stomach tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence-beta Actin antibody [1E7](ab93027)
  ICC/IF image of ab93027 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93027, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.