Overview

  • Product nameAnti-beta Actin antibody [1E7]See all beta Actin primary antibodies ...
  • Description
    Mouse monoclonal [1E7] to beta Actin
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human beta Actin protein (AAH01301) with a proprietary 26 kDa tag.

  • Positive control
    • Human stomach tissue; NIH/3T3, Raw 264.7, Jurkat and PC 12 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab93027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [1E7] images

  • All lanes : Anti-beta Actin antibody [1E7] (ab93027) at 5 µg/ml

    Lane 1 : recombinant protein (immunogen) at 0.2 µg
    Lane 2 : PC 12 cell lysate at 50 µg
    Lane 3 : Raw 264.7 cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg
    Lane 5 : NIH/3T3 cell lysate at 50 µg


    Predicted band size : 42 kDa
  • ab93027, at 3 µg/ml, staining beta Actin in formalin fixed, paraffin embedded Human stomach tissue by Immunohistochemistry.
  • ICC/IF image of ab93027 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93027, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-beta Actin antibody [1E7] (ab93027)

ab93027 has not yet been referenced specifically in any publications.

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