Overview
- Product nameAnti-beta Actin antibody [4E8H3]See all beta Actin primary antibodies ...
- DescriptionMouse monoclonal [4E8H3] to beta Actin
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant full length protein (Human)
- Positive controlHL60 or Jurkat cell line lysates
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityProtein G purified
- Purification notesPurified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Clonality Monoclonal
- Clone number4E8H3
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab75480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000 - 1/10000. Predicted molecular weight: 42 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Omim: 102630 Human
- SwissProt: P60709 Human
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A26C1A antibodyA26C1B antibodyACTB antibody
- ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [4E8H3] images
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![Western blot - beta Actin antibody [4E8H3] (ab75480)](http://a.abcam.com/ps/datasheet/Images/75/ab75480/ab75480_1.jpg)
Western blot - beta Actin antibody [4E8H3] (ab75480)Anti-beta Actin antibody [4E8H3] (ab75480) at 1/2000 dilution + HL60 cell line lysate at 35 µg
Predicted band size : 42 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?) -
![Western blot - beta Actin antibody [4E8H3] (ab75480)](http://a.abcam.com/ps/datasheet/Images/75/ab75480/ab75480_2.jpg)
Western blot - beta Actin antibody [4E8H3] (ab75480)Anti-beta Actin antibody [4E8H3] (ab75480) at 1/2000 dilution + Jurkat cell line lysate at 35 µg
Predicted band size : 42 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
References for Anti-beta Actin antibody [4E8H3] (ab75480)
ab75480 has not yet been referenced specifically in any publications.
