Anti-beta Actin antibody [8H10D10] (ab101173)

Overview

• Product nameAnti-beta Actin antibody [8H10D10]See all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [8H10D10] to beta Actin
• Tested applicationsWB, ICC/IF, Flow Cyt, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Hamster, Human, Monkey
  
• Immunogen

  Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

• Positive controlNIH/3T3, Jurkat, Hela, CHO, PC12, HEK293, COS, A549 or MCF-7 cell lysates

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites fluid
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number8H10D10
• IsotypeIgG2b
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P60711 Rat
  • Unigene: 520640 Human

  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A26C1A antibodyA26C1B antibodyACTB antibody

  ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody [8H10D10] images

• 

  Western blot - beta Actin antibody [8H10D10] (ab101173)
  All lanes : Anti-beta Actin antibody [8H10D10] (ab101173)
  
  Lane 1 : NIH/3T3
  Lane 2 : Jurkat
  Lane 3 : HeLa
  Lane 4 : CHO
  Lane 5 : PC12
  Lane 6 : HEK293
  Lane 7 : COS
  Lane 8 : A549
  Lane 9 : MCF-7
  
  
  Predicted band size : 42 kDa
  Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [8H10D10] (ab101173)
  ab101173 staining beta Actin in SKBR-3 (left) and A549 (right) cells by Immunocytochemistry/Immunofluorescence. A Cy3-conjugated Goat polyclonal to Mouse IgG was used as the secondary antibody. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.
• 

  Flow Cytometry - beta Actin antibody [8H10D10] (ab101173)
  ab101173 staining beta Actin in MCF-7 cells (right) against staining using a negative control (left)