The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
1/200 - 1/1000.
1/200 - 1/400. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
ab101173 staining beta Actin in SKBR-3 (left) and A549 (right) cells by Immunocytochemistry/Immunofluorescence. A Cy3-conjugated Goat polyclonal to Mouse IgG was used as the secondary antibody. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.