Overview

  • Product nameAnti-beta Actin antibody [8H10D10]See all beta Actin primary antibodies ...
  • Description
    Mouse monoclonal [8H10D10] to beta Actin
  • Tested applicationsWB, ICC/IF, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Monkey
  • Immunogen

    Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

  • Positive control
    • NIH/3T3, Jurkat, Hela, CHO, PC12, HEK293, COS, A549 or MCF-7 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab101173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.
ELISA 1/10000.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [8H10D10] images

  • All lanes : Anti-beta Actin antibody [8H10D10] (ab101173)

    Lane 1 : NIH/3T3
    Lane 2 : Jurkat
    Lane 3 : HeLa
    Lane 4 : CHO
    Lane 5 : PC12
    Lane 6 : HEK293
    Lane 7 : COS
    Lane 8 : A549
    Lane 9 : MCF-7


    Predicted band size : 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • ab101173 staining beta Actin in SKBR-3 (left) and A549 (right) cells by Immunocytochemistry/Immunofluorescence. A Cy3-conjugated Goat polyclonal to Mouse IgG was used as the secondary antibody. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.
  • ab101173 staining beta Actin in MCF-7 cells (right) against staining using a negative control (left)

References for Anti-beta Actin antibody [8H10D10] (ab101173)

This product has been referenced in:
  • Huang L  et al. Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome. PLoS One 9:e88893 (2014). WB . Read more (PubMed: 24586430) »
  • Zhang SQ  et al. Exome sequencing identifies MVK mutations in disseminated superficial actinic porokeratosis. Nat Genet 44:1156-60 (2012). Read more (PubMed: 22983302) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"