Anti-beta Actin antibody [AC-15] (FITC) (ab6277)
Overview
- Product nameAnti-beta Actin antibody [AC-15] (FITC)See all beta Actin primary antibodies ...
- DescriptionMouse monoclonal [AC-15] to beta Actin (FITC)
- ConjugationFITC
- Conjugation notesConjugated to FITC
- Tested applicationsICC/IF, ICC, IHC-Fr, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Fruit fly (Drosophila melanogaster), Fish
- Immunogen
Synthetic peptide: DDDIAALVIDNGSGL conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).
- EpitopeThe N-terminal of the beta isoform of actin
- Positive controlCultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
- General notesImmunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.
Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPBS with 1% BSA and 0.05% sodium azide
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Concentration information loading... - PurityIgG fraction
- Primary antibody notes Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells
- Clonality Monoclonal
- Clone numberAC-15
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. PubMed: 21562077 |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent dilution. PubMed: 21445349 |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: Q6QAQ1 Pig
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: P60713 Sheep
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [AC-15] (FITC) images
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![Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)](http://a.abcam.com/ps/datasheet/Images/6/ab6277/ab6722_1.jpg)
Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x20
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![Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)](http://a.abcam.com/ps/datasheet/Images/6/ab6277/ab6722_2.jpg)
Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 no deconvolution.
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![Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)](http://a.abcam.com/ps/datasheet/Images/6/ab6277/ab6722_3.jpg)
Immunofluorescence - Anti-beta Actin antibody [AC-15] (FITC) (ab6277)ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.
30 min blocking with TBS-T / 5% BSA
1 hr incubation with ab6277 (TBS-T / 5% BSA)
1 minute nuclear counterstaining with DAPIMagnification x40 “Inverse Filter” Deconvolution.
Protocols
References for Anti-beta Actin antibody [AC-15] (FITC) (ab6277)
This product has been referenced in:
- Cheever TR et al. Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin in vivo. PLoS One 6:e17768 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21445349) »
- Parathath S et al. Diabetes adversely affects macrophages during atherosclerotic plaque regression in mice. Diabetes 60:1759-69 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21562077) »
