Anti-beta Actin antibody [AC-15] (FITC) (ab6277)

Overview

  • Product nameAnti-beta Actin antibody [AC-15] (FITC)
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [AC-15] to beta Actin (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Conjugation notesConjugated to FITC
  • Tested applicationsICC/IF, ICC, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Fruit fly (Drosophila melanogaster), Fish
  • Immunogen

    Synthetic peptide:

    DDDIAALVIDNGSGL

    conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).

  • EpitopeThe N-terminal of the beta isoform of actin
  • Positive control
    • Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
  • General notesImmunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.


    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

Properties

Applications

Our Abpromise guarantee covers the use of ab6277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent concentration. PubMed: 21562077
IHC-FoFr Use at an assay dependent dilution. PubMed: 21445349

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A X actin like protein antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin antibody
    • Actin cytoplasmic 1 antibody
    • actin, beta antibody
    • Actx antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • cytoplasmic 1 antibody
    • Melanoma X actin antibody
    • N-terminally processed antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5-binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [AC-15] (FITC) images

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x20

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40 no deconvolution.

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40  “Inverse Filter” Deconvolution.

Protocols

References for Anti-beta Actin antibody [AC-15] (FITC) (ab6277)

This product has been referenced in:
  • Perrin BJ  et al. ß-Actin and fascin-2 cooperate to maintain stereocilia length. J Neurosci 33:8114-21 (2013). Mouse . Read more (PubMed: 23658152) »
  • Cheever TR  et al. Axonal regeneration and neuronal function are preserved in motor neurons lacking ß-actin in vivo. PLoS One 6:e17768 (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21445349) »

See all 9 Publications for this product

Product Wall

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Thank you for contacting us today. I can confirm that that these images were labelled incorrectly and have been updated. ab6277 was used in all 3 images. These changes should be visible on the PWS but can take a couple of days to be refreshed. ...

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Unfortunately we are unable to provide a concentration for this antibody because it is conjugated to FITC.

This antibody has not yet been tested for use with on formalin fixed, paraffin sections of mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"