Overview

  • Product nameAnti-beta Actin antibody [AC-15] (HRP)
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [AC-15] to beta Actin (HRP)
  • ConjugationHRP
  • SpecificityThis antibody labels specifically ß-actin in a wide variety of tissues and species. It does not stain adult cardiac and skeletal muscles except for contaminants.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Monkey
    Predicted to work with: a wide range of other speciesDoes not react with: Fruit fly (Drosophila melanogaster), Dictyostelium discoideum
  • Immunogen

    Synthetic N terminal peptide:

    DDDIAALVIDNGSGK

    conjugated to KLH, corresponding 100% to a sequence from: Xenopus borealis (Kenyan clawed frog), Lytechinus pictus (Painted sea urchin) and Engystomops pustulosus (Tungara frog).

  • Positive control
    • Chicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts. Rat dorsal root ganglion and Rat liver lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab49900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Predicted molecular weight: 42 kDa.

Target

Anti-beta Actin antibody [AC-15] (HRP) images

  • All lanes : Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/50000 dilution

    Lane 1 : A549 Lysate
    Lane 2 : HeLa Lysate
    Lane 3 : 3T3 Lysate
    Lane 4 : NRK Lysate
    Lane 5 : MDCK Lysate
    Lane 6 : MDBK Lysate
    Lane 7 : BHK Lysate
    Lane 8 : COS7 Lysate
    Lane 9 : CFB Lysate

    developed using the ECL technique

    Predicted band size : 42 kDa

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400.

    Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.

    Normalization of results was ensured by running parallel Western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.

  • Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C.

    See Abreview

References for Anti-beta Actin antibody [AC-15] (HRP) (ab49900)

This product has been referenced in:
  • Ambade A  et al. Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease. World J Gastroenterol 22:4091-108 (2016). WB ; Mouse . Read more (PubMed: 27122661) »
  • Dow LE  et al. Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol 33:390-4 (2015). WB ; Mouse . Read more (PubMed: 25690852) »

See all 38 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Fat-adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2016

Application Western blot
Sample Human Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Fat-adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2016

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 08 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount 50 µg
Specification ParC5 rat parotid cell line
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 10 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 19 2010

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 30 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 21 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 20 µg
Specification 293T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"