Anti-beta Actin antibody [AC-15] (HRP) (ab49900)
Overview
- Product nameAnti-beta Actin antibody [AC-15] (HRP)See all beta Actin primary antibodies ...
- DescriptionMouse monoclonal [AC-15] to beta Actin (HRP)
- ConjugationHRP
- SpecificityThis antibody labels specifically ß-actin in a wide variety of tissues and species. It does not stain adult cardiac and skeletal muscles except for contaminants.
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Fruit fly (Drosophila melanogaster), Monkey
Predicted to work with: a wide range of other speciesDoes not react with
Dictyostelium discoideum - Immunogen
Synthetic N terminal peptide: DDDIAALVIDNGSGK conjugated to KLH, corresponding 100% to a sequence from: Xenopus borealis (Kenyan clawed frog), Lytechinus pictus (Painted sea urchin) and Engystomops pustulosus (Tungara frog).
- Positive controlChicken gizzard tissue cryosections. Human foreskin fibroblasts. Chicken fibroblasts.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 0.0268% PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- Clone numberAC-15
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab49900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/25000 - 1/50000. Predicted molecular weight: 42 kDa. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 442937 Dog
- Entrez Gene: 100135470 Guinea pig
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 443052 Sheep
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: O18840 Dog
- SwissProt: Q71FK5 Guinea pig
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: Q6QAQ1 Pig
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: P60713 Sheep
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [AC-15] (HRP) images
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![Western blot - beta Actin antibody [AC-15] (HRP) (ab49900)](http://a.abcam.com/ps/datasheet/images/49/ab49900/beta-Actin-Primary-antibodies-ab49900-2.jpg)
Western blot - beta Actin antibody [AC-15] (HRP) (ab49900)Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDaThis image is courtesy of an anonymous Abreview
References for Anti-beta Actin antibody [AC-15] (HRP) (ab49900)
This product has been referenced in:
- Baer A et al. Induction of DNA Damage Signaling upon Rift Valley Fever Virus Infection Results in Cell Cycle Arrest and Increased Viral Replication. J Biol Chem 287:7399-410 (2012). WB . Read more (PubMed: 22223653) »
- Thalhammer A et al. Human AlkB Homologue 5 Is a Nuclear 2-Oxoglutarate Dependent Oxygenase and a Direct Target of Hypoxia-Inducible Factor 1a (HIF-1a). PLoS One 6:e16210 (2011). WB ; Human . Read more (PubMed: 21264265) »
