Overview

  • Product nameAnti-beta Actin antibody [AC-15]See all beta Actin primary antibodies ...
  • Description
    Mouse monoclonal [AC-15] to beta Actin
  • Tested applicationsFlow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chinese Hamster
  • Immunogen

    Synthetic peptide corresponding to beta Actin aa 2-15 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Slightly modified beta cytoplasmic actin.
    Sequence:

    DDDIAALVIDNGSGK

Properties

Applications

Our Abpromise guarantee covers the use of ab82618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Target

Anti-beta Actin antibody [AC-15] images

  • Overlay histogram showing HeLa cells stained with ab82618 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82618, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
  • ICC/IF image of ab82618 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82618, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-beta Actin antibody [AC-15] (ab82618)

This product has been referenced in:
  • Berkenkamp B  et al. In vivo and in vitro analysis of age-associated changes and somatic cellular senescence in renal epithelial cells. PLoS One 9:e88071 (2014). WB ; Mouse . Read more (PubMed: 24505380) »
  • Abdelhamed ZA  et al. Variable expressivity of ciliopathy neurological phenotypes that encompass Meckel-Gruber syndrome and Joubert syndrome is caused by complex de-regulated ciliogenesis, Shh and Wnt signalling defects. Hum Mol Genet 22:1358-72 (2013). WB ; Mouse . Read more (PubMed: 23283079) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab82618.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"