Anti-beta Actin antibody [AC-15] (ab82618)

Overview

• Product nameAnti-beta Actin antibody [AC-15]See all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [AC-15] to beta Actin
• Tested applicationsICC/IF, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chinese Hamster
• Immunogen

  Synthetic peptide: DDDIAALVIDNGSGK (slightly modified) conjugated to KLH, corresponding to N terminal amino acids 2-15 of beta Actin

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
  Constituents: 1.2% Sodium acetate, 2mg/ml BSA
• Concentration information loading...
• Purification notesPurified by the goat anti-mouse IgG affinity chromatography
• Clonality Monoclonal
• Clone numberAC-15
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396526 Chicken
  • Entrez Gene: 280979 Cow
  • Entrez Gene: 442937 Dog
  • Entrez Gene: 100135470 Guinea pig
  • Entrez Gene: 100033878 Horse
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 414396 Pig

  • Entrez Gene: 100009272 Rabbit
  • Entrez Gene: 81822 Rat
  • Entrez Gene: 443052 Sheep
  • Omim: 102630 Human
  • SwissProt: P60706 Chicken
  • SwissProt: P60712 Cow
  • SwissProt: O18840 Dog
  • SwissProt: Q71FK5 Guinea pig
  • SwissProt: P60708 Horse
  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: Q6QAQ1 Pig
  • SwissProt: P29751 Rabbit
  • SwissProt: P60711 Rat
  • SwissProt: P60713 Sheep
  • Unigene: 520640 Human
  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A26C1A antibodyA26C1B antibodyACTB antibody

  ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody [AC-15] images

• 

  Immunocytochemistry/ Immunofluorescence-beta Actin antibody [AC-15](ab82618)
  ICC/IF image of ab82618 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82618, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.