Anti-beta Actin [AC-15] antibody (ab6276)

Overview

• Product nameAnti-beta Actin [AC-15] antibodySee all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [AC-15] to beta Actin
• SpecificityThis antibody makes a good loading control antibody and we recommend its use as such. We also recommend ab8226, another excellent mouse monoclonal beta actin loading control antibody.
• Tested applicationsICC/IF, Dot Blot, IHC-FoFr, ICC, IHC-P, IHC-Fr, Indirect ELISA, WB, ELISA, IHC-FrFl more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Fish, Opossum, Marmoset (common), Meriones unguiculatus
  

  Does not react with

  Fruit fly (Drosophila melanogaster), Dictyostelium discoideum
• Immunogen

  Synthetic peptide: DDDIAALVIDNGSGK conjugated to KLH.

  Run BLAST with Run BLAST with
• EpitopeN-terminal of the beta isoform of actin.
• Positive controlCultured human or chicken fibroblasts as described in Liao et al.
• General notesImmunofluoresence staining of chicken gizzard ultra-thin sections (North et al. J. Cell Sci. 107:445-455 (1994)) labels the dense bodies, longitudinal channels and membrane associated dense-plaque.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferAscitic fluid with 15mM sodium azide
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone numberAC-15
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396526 Chicken
  • Entrez Gene: 280979 Cow
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60706 Chicken
  • SwissProt: P60712 Cow

  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P29751 Rabbit
  • SwissProt: P60711 Rat
  • SwissProt: P60713 Sheep
  • Unigene: 520640 Human
  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody

  Actin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin [AC-15] antibody images

• 

  Western blot - beta Actin antibody [AC-15] (ab6276)
  
  
  Predicted band size : 42 kDa
  
  
  MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard asssay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.

  David Grimm, Yale University, USA.

• 

  Western blot - beta Actin antibody [AC-15] (ab6276)
  All lanes : Anti-beta Actin [AC-15] antibody (ab6276) at 1/5000 dilution
  
  Lane 1 : HeLa nuclear
  Lane 2 : HeLa whole cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : Jurkat cell lysate
  Lane 5 : HEK293 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Alexa Fluor anti mouse at 1/5000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [AC-15] (ab6276)This image is courtesy of an anonymous Abreview

  ab6276 at 1/500 staining human macrophage cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. A Cy2 ® conjugated donkey antibody was used as the secondary.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Actin antibody [AC-15] (ab6276)This image is courtesy of an Abreview submitted by Mr Carl hobbs

  ab6276 at 1/10000 staining mouse brain tissue sections by IHC-P. The mouse brain was immersion-fixed in 4% Formalin/PBS for 2 days, then bisected mid-saggitally. The tissue was further fixed in same solution for a further 3 days. Both halves were processed to pwax on a dip-n-dunk tissue processor using a 20hr-cycle. Sections were cut at 5microns,floated out on water at 47C, mounted on Superfrost Plus slides and dried/melted for 24hrs in a 62C oven.

  The whole brain shows positivity but there are areas/cells that are enriched. Note that the axonal tracks are negatively stained at this dilution factor, yet some cells within those tracks are still strongly positive (many similar cells outside of the tracks are also positive).

  See Abreview

• 

  Western blot - beta Actin antibody [AC-15] (ab6276)
  
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  

  This image is courtesy of an Abreview submitted by Dr Mark Elliott

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [AC-15] (ab6276)

  ab6276 staining beat actin in rat hypothalamus primary cells by ICC/IF. The cells were formaldehyde fixed, permeabilized in 0.5% Triton X-100 and blocked in 5% serum for 20 minutes at 25°C. The primary antibody was diluted 1/1000 in PBS (0.1% Triton X-100, 1% goat serum) and incubated with sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat polyclonal to mouse IgG1, diluted 1/500 was used as secondary.
  

  See Abreview

• 

  ELISA - beta Actin antibody [AC-15] (ab6276)This image is courtesy of an anonymous Abreview
  ab6276 detecting beta actin in AGS Human gastric carcinoma cells by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. Ab6729 (1/1000) was used as the secondary antibody.

  See Abreview