Anti-beta Actin antibody [BA3R] (ab92436)

Overview

• Product nameAnti-beta Actin antibody [BA3R]See all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [BA3R] to beta Actin
• SpecificityRecognizes native and denatured forms of ß-Actin (~42kDa)
• Tested applicationsICC, ELISA, Dot Blot, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  Predicted to work with: Sheep, Horse, Guinea pig, Hamster, Cow, Pig, Chimpanzee, Cynomolgus Monkey, Japanese ricefish, Orangutan
• Immunogen

  Synthetic peptide corresponding to amino acids 2 - 15 (DDDIAALVVDNGSG) of Human beta Actin cytoplasmic domain (slightly modified)

  Run BLAST with Run BLAST with
• Positive control293 cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.20
  Preservative: 0.05% Sodium azide
  Constituent: 0.0268% PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone numberBA3R
• IsotypeIgG2b
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396526 Chicken
  • Entrez Gene: 45013 Chimpanzee
  • Entrez Gene: 280979 Cow
  • Entrez Gene: 100135470 Guinea pig
  • Entrez Gene: 100033878 Horse
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 414396 Pig

  • Entrez Gene: 100009272 Rabbit
  • Entrez Gene: 81822 Rat
  • Entrez Gene: 443052 Sheep
  • Omim: 102630 Human
  • SwissProt: P60706 Chicken
  • SwissProt: Q5R1X3 Chimpanzee
  • SwissProt: P60712 Cow
  • SwissProt: Q71FK5 Guinea pig
  • SwissProt: P60708 Horse
  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: Q6QAQ1 Pig
  • SwissProt: P29751 Rabbit
  • SwissProt: P60711 Rat
  • SwissProt: P60713 Sheep
  • Unigene: 520640 Human
  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody

  Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody [BA3R] images

• 

  Western blot - Anti-beta Actin antibody [BA3R] (ab92436)
  All lanes : Anti-beta Actin antibody [BA3R] (ab92436) at 1/1000 dilution
  
  Lane 1 : Human at 20 µg/ml
  Lane 2 : Mouse at 20 µg/ml
  Lane 3 : Rat at 20 µg/ml
  Lane 4 : Rabbit at 20 µg/ml
  
  
  Predicted band size : 42 kDa
  
• 

  Western blot - beta Actin antibody [BA3R] (ab92436)
  All lanes : Anti-beta Actin antibody [BA3R] (ab92436) at 1/1000 dilution
  
  Lane 1 : 293 cell lysate at 3 µg
  Lane 2 : 293 cell lysate at 10 µg
  Lane 3 : 293 cell lysate at 30 µg
  
  
  Predicted band size : 42 kDa