(1854-1)
Anti-beta Actin antibody [EP1123Y] (ab52614)
Overview
- Product nameAnti-beta Actin antibody [EP1123Y]See all beta Actin primary antibodies ...
- DescriptionRabbit monoclonal [EP1123Y] to beta Actin
- Tested applicationsICC/IF, WB, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin).
- Positive controlT47D, HeLa cell lysate, human muscle tissue, NIH/3T3 cells
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEP1123Y
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab52614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/50. |
| WB | WB: 1/500. Predicted molecular weight: 42 kDa. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P60711 Rat
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A26C1A antibodyA26C1B antibodyACTB antibody
- ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodybeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [EP1123Y] images
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![Western blot - beta Actin antibody [EP1123Y] (ab52614)](http://a.abcam.com/ps/datasheet/Images/52/ab52614/ab52614wb.jpg)
Western blot - beta Actin antibody [EP1123Y] (ab52614)All lanes : Anti-beta Actin antibody [EP1123Y] (ab52614) at 1/500 dilution
Lane 1 : T47D cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa -
![Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y] (ab52614)](http://a.abcam.com/ps/datasheet/Images/52/ab52614/ab52614ihc.jpg)
Immunohistochemistry (Paraffin-embedded sections) - beta Actin antibody [EP1123Y] (ab52614)Ab52614 at 1/50 dilution staining human muscle; paraffin embedded.
References for Anti-beta Actin antibody [EP1123Y] (ab52614)
This product has been referenced in:
- Lupberger J et al. EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy. Nat Med 17:589-95 (2011). WB ; Human . Read more (PubMed: 21516087) »
- Hirooka S et al. Localization of the invadopodia-related proteins actinin-1 and cortactin to matrix-contact-side cytoplasm of cancer cells in surgically resected lung adenocarcinomas. Pathobiology 78:10-23 (2011). Read more (PubMed: 21474972) »
