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Products:Isotype/Loading Controls >> Loading Controls >> Beta Actin
Anti-beta Actin antibody - Loading Control (ab13822)
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Proteins and Peptides
ab13772
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - beta Actin antibody - Loading Control (ab13822)
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1/1000 dilution
Lane 1 : HeLa cells (Human) at 20 µg
Lane 2 : 3T3 cells (Mouse) at 20 µg
Lane 3 : Rat Liver at 20 µg
Lane 4 : Fish Liver at 20 µg
Lane 5 : Rabbit Liver at 20 µg
Lane 6 : MDCK cells (Dog) at 20 µg
Lane 7 : EBTr cells (Cow) at 20 µg
Lane 8 : SL-29 cells (Chicken) at 20 µg
Lane 9 : CHO cells (Chinese Hamster) at 20 µg
Lane 10 : Xenopus embryos
Lane 11 : Zebrafish embryos
Secondary
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 41 kDa
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - beta Actin antibody - Loading Control (ab13822)
ICC/IF image of ab13822 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab13822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
beta Actin antibody - Loading Control for WB in Rat (13822)
beta Actin antibody - Loading Control for Western blot in Mouse (13822)
beta Actin antibody - Loading Control for WB in Human (13822)
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