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Products:Isotype/Loading Controls >> Loading Controls >> Beta Actin
Overview
Product name
Anti-beta Actin antibody - Loading Control
See all beta Actin products (9) ...
Description
Chicken polyclonal to beta Actin - Loading Control
Specificity
Detects a single clean band at 40kD representing beta actin. Does not cross-react with adult cardiac, smooth or skeletal muscle actin.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Xenopus laevis, Fish, Chinese Hamster
Does not react with
Zebrafish
Immunogen
Synthetic peptide derived from residues 1 - 100 of Human beta Actin.
.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 3% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/1000Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similarities
Belongs to the actin family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accessionP60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic 1 antibody
- N-terminally processed antibody
- A X actin like protein antibody
see all
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 398459 Xenopus laevis
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
see all
Anti-beta Actin antibody - Loading Control images:
Western blot - beta Actin antibody - Loading Control (ab13822)
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1/1000 dilution
Lane 1 : HeLa cells (Human) at 20 µg
Lane 2 : 3T3 cells (Mouse) at 20 µg
Lane 3 : Rat Liver at 20 µg
Lane 4 : Fish Liver at 20 µg
Lane 5 : Rabbit Liver at 20 µg
Lane 6 : MDCK cells (Dog) at 20 µg
Lane 7 : EBTr cells (Cow) at 20 µg
Lane 8 : SL-29 cells (Chicken) at 20 µg
Lane 9 : CHO cells (Chinese Hamster) at 20 µg
Lane 10 : Xenopus embryos
Lane 11 : Zebrafish embryos
Secondary
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6877) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 41 kDa
Exposure time : 1 minute
Immunocytochemistry/ Immunofluorescence - beta Actin antibody - Loading Control (ab13822)
ICC/IF image of ab13822 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab13822, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-chicken IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-beta Actin antibody - Loading Control (ab13822)
This product has been referenced in:
- Xu Yet al. Expression, functional, and structural analysis of proteins critical for otoconia development. Dev Dyn 239:2659-73 (2010). WB; Mouse.Read more (PubMed: 20803598) »
- Jackson TCet al. Regional hippocampal differences in AKT survival signaling across the lifespan: implications for CA1 vulnerability with aging. Cell Death Differ 16:439-48 (2009). WB; Rat.Read more (PubMed: 19039330) »
See all 3 publications for this product
Publishing research using ab13822? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"