You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-375 of Human beta Actin (NP_001092.1).

  • Positive controlMouse intestine tissue lysate and 293T cell lysate transfected with beta Actin.

Properties

Applications

Our Abpromise guarantee covers the use of ab103548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 42 kDa.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      A26C1A antibodyA26C1B antibodyACTB antibody
      ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images

  • Western blot - beta Actin antibody (ab103548)
    Western blot - beta Actin antibody (ab103548)
    Anti-beta Actin antibody (ab103548) at 1/500 dilution + Mouse intestine tissue lysate at 50 µg

    Predicted band size : 42 kDa
  • Western blot - beta Actin antibody (ab103548)
    Western blot - beta Actin antibody (ab103548)
    All lanes : Anti-beta Actin antibody (ab103548) at 1/500 dilution

    Lane 1 : beta Actin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 42 kDa

References for Anti-beta Actin antibody (ab103548)

ab103548 has not yet been referenced specifically in any publications.

Publishing research using ab103548 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab103548 Submit a Question for ab103548

There are currently no Abreviews or Questions for ab103548.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"