Overview
- Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
- DescriptionRabbit polyclonal to beta Actin
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Fruit fly (Drosophila melanogaster) - Immunogen
Synthetic peptide: APRAVFPS, corresponding to amino acids 26-33 of beta Actin.
- Positive controlSkin and leiomyoma
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab16039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
| IHC-P | IHC-P: 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 442937 Dog
- Entrez Gene: 100135470 Guinea pig
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 443052 Sheep
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: O18840 Dog
- SwissProt: Q71FK5 Guinea pig
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: Q6QAQ1 Pig
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: P60713 Sheep
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A26C1A antibodyA26C1B antibodyACTB antibody
- ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodybeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Actin antibody (ab16039)ab16039 at 1/100 dilution staining human beta Actin in Leiomyoma by Immunohistochemistry (FFPE-sections) -
Western blot - Anti-beta Actin antibody (ab16039)All lanes : Anti-beta Actin antibody (ab16039) at 1 µg/ml
Lane 1 : SK N SH (Human neuroblastoma) Whole Cell Lysate (ab76277)
Lane 2 :Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate (ab31423)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
References for Anti-beta Actin antibody (ab16039)
This product has been referenced in:
- Halperin J et al. Prolactin signaling through the short form of its receptor represses forkhead transcription factor FOXO3 and its target gene galt causing a severe ovarian defect. Mol Endocrinol 22:513-22 (2008). WB ; Human . Read more (PubMed: 17975019) »
- Lisse TS et al. ER stress-mediated apoptosis in a new mouse model of osteogenesis imperfecta. PLoS Genet 4:e7 (2008). WB ; Mouse . Read more (PubMed: 18248096) »
