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Synthetic peptide: APRAVFPS, corresponding to amino acids 26-33 of beta Actin.
Our Abpromise guarantee covers the use of ab16039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|IP||Use a concentration of 5 µg/ml.|
ab16039 at 1/100 dilution staining Human beta Actin in Leiomyoma by Immunohistochemistry (FFPE-sections)
beta Actin was immunoprecipitated using 0.5mg SK-N-SH whole cell extract, 5µg of Rabbit polyclonal to beta Actin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42kDa; beta Actin
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