Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. Ab25894 was raised against a 16 amino acid peptide from near the carboxy-terminus of human ß-actin.

  • Positive control
    • HeLa cell lysate can be used as positive control.

Properties

Applications

Our Abpromise guarantee covers the use of ab25894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b-actin antibody
    • beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images



  • Predicted band size : 42 kDa
  • ab25894 at 10µg/ml staining beta Actin in Hela cells by ICC/IF

  • ab25894 (2µg/ml) staining beta actin in human placenta.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-beta Actin antibody (ab25894)

This product has been referenced in:
  • Jian B  et al. Resveratrol restores sirtuin 1 (SIRT1) activity and pyruvate dehydrogenase kinase 1 (PDK1) expression after hemorrhagic injury in a rat model. Mol Med 20:10-6 (2014). WB . Read more (PubMed: 24395567) »
  • Wang L  et al. Regulation of the phosphorylation and nuclear import and export of ß-catenin by APC and its cancer-related truncated form. J Cell Sci 127:1647-59 (2014). Read more (PubMed: 24496450) »

See all 4 Publications for this product

Product Wall

Thank you for calling Abcam earlier today.

I am sorry that you are some trouble with ab25894 in western blot. As we discussed on the phone, I am sending you a new vial of antibody to try out. Your new order number is *****.

If there...

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Thanks for your patience.

I have sent you ab23750 & ab8227 free of charge replacements for ab6328 & ab25894, from the original order number ***. The order/reference number for these is ****

Also as a goodwill gesture, I am s...

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Thank you for that information.

The order number you provided has the information for ab6328 and ab25894 but not ab11575 (Anti-Laminin antibody). I have searched through the order history for this antibody and I cannot find the order that you...

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Thank you for your reply.

For ab30320 and ab23750, I do not have access to the exact immunogen sequence but there has to be a minimum of 85% homology between the immunogen sequence and the target species before we will 'predict' it to work. <...

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Thank you for your reply.

I agree that in the western blot, a concentration of 1/100, with 20-25ug total protein and an overnight incubation at 4C, should be enough to see even a faint signal. For the IHC-P, 1/100 may have been too dilute but...

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Thank you for contacting Abcam.

I am sorry that you are having so many problems with our antibodies.

Under our Abpromise we guarantee all of those antibodies for 6 months to work in IHC-P and western blot in both mouse and rat. If w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"