Anti-beta Actin antibody (ab34731)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab34731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/200 - 1/1000.
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Detects a band of approximately 42 kDa.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actx antibody
    • beta cytoskeletal actin antibody
    • Beta-actin antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images

  • All lanes : Anti-beta Actin antibody (ab34731) at 1/1500 dilution

    Lane 2 : Human embryonic kidney 293
    Lane 3 : Human lung carcinoma A549
    Lane 4 : Mouse brain

    Lysates/proteins at 35 µg per lane.


    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining using ab34731 antibody at 1/200 dilution shows detection of actin at the neuromuscular juntion of rana pipiens tissue fixed in 4% paraformaldehyde using a section 4.2 µm thick.

References for Anti-beta Actin antibody (ab34731)

ab34731 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Mouse Recombinant protein (Recombinant ActB)
Specification Recombinant ActB
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type Indirect
Username

Abcam user community

Verified customer

Submitted Apr 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 5 µg
Specification MEF
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 24 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"