Overview
- Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
- DescriptionRabbit polyclonal to beta Actin
- Tested applicationsIHC-P, ELISA, WB more details
- Species reactivityReacts with a wide range of species.
- Immunogen
Synthetic peptide: TFQQMWISKQ, corresponding to amino acids 359-368 of beta Actin
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab34731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/200 - 1/1000. |
| ELISA | ELISA: 1/10000 - 1/50000. |
| WB | WB: 1/500 - 1/3000. Detects a band of approximately 42 kDa. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A26C1A antibodyA26C1B antibodyACTB antibody
- ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodybeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
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Anti-beta Actin antibody images
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Western blot - beta Actin antibody (ab34731)All lanes : Anti-beta Actin antibody (ab34731) at 1/1500 dilution
Lane 2 : Human embryonic kidney 293
Lane 3 : Human lung carcinoma A549
Lane 4 : Mouse brain
Lysates/proteins at 35 µg per lane.
Observed band size : 42 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta Actin antibody (ab34731)Immunohistochemical staining using ab34731 antibody at 1/200 dilution shows detection of actin at the neuromuscular juntion of rana pipiens tissue fixed in 4% paraformaldehyde using a section 4.2 µm thick.
References for Anti-beta Actin antibody (ab34731)
ab34731 has not yet been referenced specifically in any publications.
