Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • SpecificityPreliminary indications are that this antibody also recognizes gamma Actin as the N-terminus of beta Actin is highly conserved with respect to gamma Actin.
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish
  • Immunogen

    The N-terminus of human Beta-actin.

  • Positive control
    • NIH3T3 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Antigen retrieval is not essential but may optimise staining.
WB 1/5000 - 1/15000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

Target

Anti-beta Actin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling beta Actin with ab70165 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling beta Actin with ab70165 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-beta Actin antibody (ab70165) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    developed using the ECL technique

    Predicted band size : 42 kDa


    Exposure time : 30 seconds
  • All lanes : Anti-beta Actin antibody (ab70165) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 2.5 µg
    Lane 5 : HeLa whole cell lysate at 1 µg
    Lane 6 : NIH3T whole cell lysate at 50 µg


    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-beta Actin antibody (ab70165)

ab70165 has not yet been referenced specifically in any publications.

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