Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • SpecificityPreliminary indications are that this antibody also recognizes gamma Actin as the N-terminus of beta Actin is highly conserved with respect to gamma Actin.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish
  • Immunogen

    The N-terminus of human Beta-actin.

  • Positive control
    • NIH3T3 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-beta Actin antibody images

    • All lanes : Anti-beta Actin antibody (ab70165) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HeLa whole cell lysate at 2.5 µg
      Lane 5 : HeLa whole cell lysate at 1 µg
      Lane 6 : NIH3T whole cell lysate at 50 µg


      Predicted band size : 42 kDa
      Observed band size : 42 kDa

    References for Anti-beta Actin antibody (ab70165)

    ab70165 has not yet been referenced specifically in any publications.

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