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Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • SpecificityPreliminary indications are that this antibody also recognizes gamma Actin as the N-terminus of beta Actin is highly conserved with respect to gamma Actin.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The N-terminus of human Beta-actin.

  • Positive controlNIH3T3 and HeLa whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/5000 - 1/15000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
    • Sequence similaritiesBelongs to the actin family.
    • Post-translational
      modifications      ISGylated.
    • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

      • Target information above from: UniProt accession P60709 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        A26C1A antibodyA26C1B antibodyACTB antibody
        ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
      see all

    Anti-beta Actin antibody images

    • Western blot - beta Actin antibody (ab70165)
      Western blot - beta Actin antibody (ab70165)
      All lanes : Anti-beta Actin antibody (ab70165) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HeLa whole cell lysate at 2.5 µg
      Lane 5 : HeLa whole cell lysate at 1 µg
      Lane 6 : NIH3T whole cell lysate at 50 µg


      Predicted band size : 42 kDa
      Observed band size : 42 kDa

    References for Anti-beta Actin antibody (ab70165)

    ab70165 has not yet been referenced specifically in any publications.

    Publishing research using ab70165 ? Please let us know so that we can cite the reference in this datasheet

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