Overview
- Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
- DescriptionGoat polyclonal to beta Actin
- SpecificityThis antibody detects a single clean band at just above 40kD that corresponds to beta Actin.
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Fish, Monkey, Chinese Hamster
Predicted to work with: a wide range of other species - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
(Peptide available as ab13772.)
- Positive controlIn Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3; PC12; CHOK1.
- General notes
This antibody has been designed for use as a loading control and is ideal for this purpose.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Primary antibody notes This antibody has been designed for use as a loading control and is ideal for this purpose.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab8229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| WB | WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with beta Actin peptide (ab13772). |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody images
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Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab8229)ICC/IF image of ab8229 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab8229, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-beta Actin antibody (ab8229)Lanes 1 - 4 : Anti-beta Actin antibody (ab8229) at 1 µg/ml (Blocked with 5% BSA.)
Lanes 5 - 8 : Anti-beta Actin antibody (ab8229) at 1 µg/ml (Blocked with 3% Milk.)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : CHO-K1 cell lysate Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : CHO-K1 cell lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes -
Western blot - Anti-beta Actin antibody (ab8229)All lanes : Anti-beta Actin antibody (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 5 minutes
References for Anti-beta Actin antibody (ab8229)
This product has been referenced in:
- Wu JQ et al. Tcf7 is an important regulator of the switch of self-renewal and differentiation in a multipotential hematopoietic cell line. PLoS Genet 8:e1002565 (2012). WB ; Mouse . Read more (PubMed: 22412390) »
- Migliorini D et al. Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice. J Clin Invest 121:1329-43 (2011). WB ; Mouse . Read more (PubMed: 21403399) »
