|
|
|
|
Western blot - beta Actin antibody - Loading Control (ab8229)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab8229)
(enlarge)
|
|
|
|
|
Product Name
|
beta Actin antibody
|
| |
See all beta Actin - Loading Control antibodies (8)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to beta Actin
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
(Peptide available as ab13772.)
|
|
Reacts with
|
Hu, Ms, Rat, Chk, ChHm, Cow, Dog, Fsh, Mk, Rb
|
|
Predicted to react
(species key)
|
Many
(Due to sequence homology)
|
|
Specificity
|
This antibody detects a single clean band at just above 40kD that corresponds to beta Actin.
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Can be blocked with beta Actin peptide (ab13772).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin
|
|
Relevance
|
Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90%".
This antibody has been designed for use as a loading control and is ideal for this purpose.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.025% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this beta Actin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for beta Actin antibody images, references, products related to ab8229 and other tools.
|
| |
beta Actin antibody images:
|
 Western blot - beta Actin antibody - Loading Control (ab8229)
All lanes : beta Actin antibody (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab6741) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 5 minutes
|
| |
Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab8229)
ICC/IF image of ab8229 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab8229, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
|
| |
References for beta Actin antibody (ab8229)
|
|
This product has been used in: (two most recent references)
Tskitishvili E et al. Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uterus. Am J Reprod Immunol 63:137-43 (2010). WB; Mouse. PubMed: 20015327
Kirat D et al. Dietary pectin up-regulates monocaboxylate transporter 1 in the rat gastrointestinal tract. Exp Physiol 94:422-33 (2009). PubMed: 19168540
See all 13 publication references for this product.
If you publish research using ab8229 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to beta Actin
|
| |
beta Actin antibody - more information
|
Customer reviews (feedback) regarding beta Actin antibody |
Customer FAQs regarding beta Actin antibody |
Protocols for beta Actin antibody |
Price and availability of products related to beta Actin antibody |
| |
beta Actin antibody - related products:
|
|
|
| |
beta Actin antibody - other tools:
|
Contact Abcam with a Technical Enquiry about beta Actin antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
