Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Isotype/Loading Controls >> Loading Controls >> Beta Actin
Overview
Product name
Anti-beta Actin antibody
See all beta Actin products (9) ...
Description
Goat polyclonal to beta Actin
Specificity
This antibody detects a single clean band at just above 40kD that corresponds to beta Actin.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Fish, Monkey, Chinese Hamster
Predicted to work with
a wide range of other species
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
(Peptide available as ab13772.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody has been designed for use as a loading control and is ideal for this purpose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/500Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with beta Actin peptide (ab13772).
Target
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similarities
Belongs to the actin family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Target information above from: UniProt accessionP60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cytoplasmic 1 antibody
- N-terminally processed antibody
- A X actin like protein antibody
see all
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: P60709 Human
see all
Anti-beta Actin antibody images:
Western blot - beta Actin antibody - Loading Control (ab8229)
All lanes : Anti-beta Actin antibody (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 5 minutes
Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab8229)
ICC/IF image of ab8229 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab8229, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal donkey serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-beta Actin antibody (ab8229)
This product has been referenced in:
- Migliorini Det al. Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice. J Clin Invest 121:1329-43 (2011). WB; Mouse.Read more (PubMed: 21403399) »
- Thorne JLet al. Epigenetic control of a VDR-governed feed-forward loop that regulates p21(waf1/cip1) expression and function in non-malignant prostate cells. Nucleic Acids Res 39:2045-56 (2011). WB; Mouse.Read more (PubMed: 21088000) »
See all 18 publications for this product
Publishing research using ab8229? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"