Overview

  • Product nameAnti-beta Actin antibody
    See all beta Actin primary antibodies
  • Description
    Chicken polyclonal to beta Actin
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid synthetic peptide from near the carboxy-terminus of human beta Actin (AAH02409).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A X actin like protein antibody
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • cytoplasmic 1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • N-terminally processed antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images

  • Lane 1 : Anti-beta Actin antibody (ab83955) at 1 µg/ml
    Lane 2 : Anti-beta Actin antibody (ab83955) at 2 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa

References for Anti-beta Actin antibody (ab83955)

ab83955 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 1 cells
Gel Running Conditions Non-reduced Denaturing
Sample Chinese Hamster Cell lysate - whole cell (CHO-K1)
Specification CHO-K1
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Submitted Nov 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"