Overview
- Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
- DescriptionChicken polyclonal to beta Actin
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A 16 amino acid synthetic peptide from near the carboxy-terminus of human beta Actin (AAH02409).
- Positive controlHeLa cell lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgY
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab83955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P60711 Rat
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody images
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Western blot - beta Actin antibody (ab83955)Lane 1 : Anti-beta Actin antibody (ab83955) at 1 µg/ml
Lane 2 : Anti-beta Actin antibody (ab83955) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 42 kDa
Observed band size : 42 kDa
References for Anti-beta Actin antibody (ab83955)
ab83955 has not yet been referenced specifically in any publications.
