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Overview

  • Product nameAnti-beta Actin antibodySee all beta Actin primary antibodies ...
  • Description
    Rabbit polyclonal to beta Actin
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 359 and 368 of Human beta Actin (NP_001092).

  • Positive controlNT2D1, IMR32, U87-MG, MCF-7 cell lines. A431 and H1299 whole cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab97376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/100 - 1/200.
WB WB: 1/500 - 1/3000. Predicted molecular weight: 42 kDa.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        A X actin like protein antibodyA26C1A antibodyA26C1B antibody
        ACTB antibodyACTB_HUMAN antibodyActin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodycytoplasmic 1 antibodyE430023M04Rik antibodyMelanoma X actin antibodyMGC128179 antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
      see all

    Anti-beta Actin antibody images

    • Western blot - beta Actin antibody (ab97376)
      Western blot - beta Actin antibody (ab97376)
      All lanes : Anti-beta Actin antibody (ab97376) at 1/1000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : H1299 whole cell lysate

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 42 kDa
    • Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab97376)
      Immunocytochemistry/ Immunofluorescence - beta Actin antibody (ab97376)
      Immunofluorescence analysis of methanol-fixed HeLa, using beta Actin antibody (ab97376) at 1/300 dilution.

    References for Anti-beta Actin antibody (ab97376)

    ab97376 has not yet been referenced specifically in any publications.

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