Overview

  • Product nameAnti-beta Actin antibody
    See all beta Actin primary antibodies
  • Description
    Rabbit polyclonal to beta Actin
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 359 and 368 of Human beta Actin (NP_001092).

  • Positive control
    • NT2D1, IMR32, U87-MG, MCF-7 cell lines. A431 and H1299 whole cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab97376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.

Target

  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • A X actin like protein antibody
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • cytoplasmic 1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • N-terminally processed antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody images

  • All lanes : Anti-beta Actin antibody (ab97376) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 42 kDa
  • Immunofluorescence analysis of methanol-fixed HeLa, using beta Actin antibody (ab97376) at 1/300 dilution.

References for Anti-beta Actin antibody (ab97376)

ab97376 has not yet been referenced specifically in any publications.

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