Overview

  • Product nameAnti-beta Actin antibody [mAbcam 8226] (FITC)
    See all beta Actin primary antibodies
  • Description
    Mouse monoclonal [mAbcam 8226] to beta Actin (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityDoes not cross-react with adult cardiac, smooth, or skeletal muscle actin.
  • Tested applicationsICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, African Green Monkey, Chinese Hamster
    Predicted to work with: Sheep, Horse, Guinea pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

  • General notesThe concentration of this antibody is batch dependent.

Properties

Applications

Our Abpromise guarantee covers the use of ab64496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
  • Application notesICC/IF: Use at a concentration of 1 µg/ml. FITC conjugated version of ab8226.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-beta Actin antibody [mAbcam 8226] (FITC) images

    • ICC/IF image of ab64496 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64496, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    References for Anti-beta Actin antibody [mAbcam 8226] (FITC) (ab64496)

    This product has been referenced in:
    • Mendel TA  et al. Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy. PLoS One 8:e65691 (2013). Flow Cyt ; Human . Read more (PubMed: 23741506) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"