Anti-beta Actin antibody [mAbcam 8226] (FITC) (ab64496)

Overview

• Product nameAnti-beta Actin antibody [mAbcam 8226] (FITC)See all beta Actin primary antibodies ...
• Description
  Mouse monoclonal [mAbcam 8226] to beta Actin (FITC)
• ConjugationFITC
• SpecificityDoes not cross-react with adult cardiac, smooth, or skeletal muscle actin.
• Tested applicationsICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, African Green Monkey, Chinese Hamster
  Predicted to work with: Sheep, Horse, Guinea pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

  Read Abcam's proprietary immunogen policy

• General notesThe concentration of this antibody is batch dependent.

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numbermAbcam 8226
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Isotype/Loading Controls
  • Loading Controls
  • Beta Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 100009272 Rabbit
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60706 Chicken
  • SwissProt: P60712 Cow
  • SwissProt: P60708 Horse

  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P29751 Rabbit
  • SwissProt: P60711 Rat
  • SwissProt: P60713 Sheep
  • Unigene: 520640 Human
  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody

  Actin antibodyActin cytoplasmic 1 antibodyActx antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody [mAbcam 8226] (FITC) images

• 

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] (ab64496)
  ICC/IF image of ab64496 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab64496, 1µg/ml, FITC conjugated (green)) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).