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Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

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Overview

Product name

Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control
See all beta Actin products (9) ...

Description

Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control (HRP)

Conjugation

HRP

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Fruit fly (Drosophila melanogaster), African Green Monkey, Chinese Hamster

Predicted to work with

Sheep, Horse, Guinea pig

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

Read Abcam's proprietary immunogen policy

Positive control

HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

mAbcam 8226

Myeloma

Sp2/0-Ag14

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin

  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) image (enlarge)

  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272) image (enlarge)

  • Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide10 Images

    WB

    Five stars (9 Abreviews) WB: 1/5000Detects a band...Read more →

    WB: 1/5000Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).(Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.)

Target

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Involvement in disease

Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.

Sequence similarities

Belongs to the actin family.

Post-translational
modifications

ISGylated.

Cellular localization

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accessionP60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • cytoplasmic 1 antibody
  • N-terminally processed antibody
  • A X actin like protein antibody
  • ACTB antibody
  • ACTB_HUMAN antibody
  • Actin antibody
  • Actin cytoplasmic 1 antibody
  • Actx antibody
  • Beta cytoskeletal actin antibody
  • Beta-actin antibody
  • Melanoma X actin antibody
  • PS1TP5 binding protein 1 antibody
  • PS1TP5BP1 antibody
see all

Database links

see all

Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control images:

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)

Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)

All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution

Lane 1 : human testis lysate
Lane 2 : human brain lysate
Lane 3 : human tonsil lysate
Lane 4 : mouse brain lysate
Lane 5 : mouse spleen lysate

Lysates/proteins at 20 µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42 kDa


Exposure time : 2 minutes

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)

Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)

All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : 3T3 whole cell lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa


Exposure time : 30 seconds

  Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

Lanes 1 - 4 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
Lanes 5 - 8 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa


Exposure time : 1 minute

The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

References for Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

This product has been referenced in:

  • Zimnicka AMet al. Acquisition of dietary copper: a role for anion transporters in intestinal apical copper uptake. Am J Physiol Cell Physiol 300:C588-99 (2011).Read more (PubMed: 21191107) »
  • Sanchez CGet al. Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Carcinogenesis 32:964-72 (2011). WB.Read more (PubMed: 21317300) »

See all 18 publications for this product

Publishing research using ab20272? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"