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Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

Abreviews (10)Q&A (13)Specific References (19)

Overview

  • Product nameAnti-beta Actin antibody [mAbcam 8226] (HRP) - Loading ControlSee all beta Actin primary antibodies ...
  • Description
    Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control (HRP)
  • ConjugationHRP
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Fruit fly (Drosophila melanogaster), African Green Monkey, Chinese Hamster
    Predicted to work with: Sheep, Horse, Guinea pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

    Read Abcam's proprietary immunogen policy

  • Positive controlHeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab20272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.

Target

  • Alternative names
      A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
      Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control images

  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
    Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
    All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution

    Lane 1 : human testis lysate
    Lane 2 : human brain lysate
    Lane 3 : human tonsil lysate
    Lane 4 : mouse brain lysate
    Lane 5 : mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa


    Exposure time : 2 minutes
  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
    Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)
    All lanes : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 3T3 whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 30 seconds
  • Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)
    Western blot - beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)
    Lanes 1 - 4 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
    Lanes 5 - 8 : Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) at 1/5000 dilution (Blocked in 5% MILK)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
    Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 1 minute

References for Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272)

This product has been referenced in:
  • Spindler SR  et al. Novel protein kinase signaling systems regulating lifespan identified by small molecule library screening using Drosophila. PLoS One 7:e29782 (2012). WB ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 22363408) »
  • Zimnicka AM  et al. Acquisition of dietary copper: a role for anion transporters in intestinal apical copper uptake. Am J Physiol Cell Physiol 300:C588-99 (2011). Read more (PubMed: 21191107) »

See all 19 Publications for this product

Publishing research using ab20272 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab20272 Submit a Question for ab20272

Displaying 1 - 10 of 23 results for Abreviews and Q&A

10
Votes: Highest First

Inquiry: product stopped to work, is this lot defective ? thanks Patrice

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

That appears to be a very old lot number (2008 - 2009). Do you know when you purchased the product? I'm showing most of the ex...

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Yes, I think the order you listed must be the one we placed alright.
Of the alternative antibodies you've suggested, I would prefer to try the anti-glucose-6-PDH (HRP) one (ab34601) - the fact the immunogen is the native yeast protein suggests it sho...

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Thank you for your reply.

I think that sounds like a good choice. I have arranged for the antibody (ab34601) to be sent to you free of charge. This should be with you tomorrow. This is on the order number xxxx(Purchase order number xxxxx).
...

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Thank you for getting back to me. This has been a real shame for us here - we've run numerous experiments trying to get this to work which has obviously cost us financially in terms of other consumables and indeed our time. I appreciate you offering to re...

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Thank you for confirming those details. I think I have tracked down your order. We have one for the 12th of September 2012 delivered to the following address:
xxxxx

The order number was xxxx(purchase order number xxxxx), could this be your o...

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Phone call reporting a problem in using the Anti-beta Actin antibody [mAbcam8226] (HRP) - Loading Control with yeast samples.

Thank you for contacting us yesterday in regards to the Anti-beta Actin antibody [mAbcam8226] (HRP) - Loading Control (ab20272).

I have looked into this case a little further. As we discussed over the phone, the reactivity with Saccharomyces cer...

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Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control

 Excellent
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 40 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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abreview image
Username

Verified customer

Submitted Sep 21 2012

I'm using ab20272 in WB, but seeing a doublet. I would like a replacement with ab9482.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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I have used ab20272 in the past from a different vial and it has worked well, producing one single band from cell lysates. However the most recent lot, GF58453-1, is showing multiple bands, crowded around the general vicinity of actin. Even diluting the a...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team an...

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We recently ordered two tubes of monoclonal beta-actin hrp antibody (#AB20272), and while we have had great success with this antibody in the past, our western blots with this lot had such high background that we could not detect any meaningful signal. Co...

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Thank you for contacting Abcam.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have checked our current stock, and this is the only lot currently available. I wanted to get some additional information r...

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I would like to purchase Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control (ab20272) but I have a query about it first. Can this antibody detect if the sample has been prepared using SDS or will it destroy the epitope?   Looking forward ...

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Thank you for your inquiry.

Unfortunately, we do not currently have information about use of this product with an SDS lysis buffer. However we do not believe that this type of treatment will have detrimental effects for the loading control. ...

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Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] (HRP) - Loading Control

 Excellent
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (Breast epithelial)
Loading amount 25 µg
Specification Breast epithelial
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Username

Verified customer

Submitted Nov 10 2009



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