Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
Overview
- Product nameAnti-beta Actin antibody [mAbcam 8226] - Loading ControlSee all beta Actin primary antibodies ...
- DescriptionMouse monoclonal [mAbcam 8226] to beta Actin - Loading Control
- SpecificityDoes not cross-react with adult cardiac, smooth, or skeletal muscle actin.
- Tested applicationsICC/IF, ICC, Flow Cyt, IHC-FrFl, IHC-P, IHC-Fr, IP, WB more details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, African Green Monkey, Chinese Hamster, Armenian Hamster
Predicted to work with: Sheep, Horse, Guinea pig - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
(Peptide available as ab13772.)
- General notesThe concentration of this antibody is batch dependent.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Contains 0.4M Arginine -
Concentration information loading... - PurityIgG fraction
- Clonality Monoclonal
- Clone numbermAbcam 8226
- MyelomaSp2/0-Ag14
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab8226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| ICC | ICC: Use at an assay dependent dilution. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| IHC-FrFl | IHC-FrFl: Use at an assay dependent concentration. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: 1/500. |
| IP | IP: 1/100. |
| WB | WB: 1/500 - 1/10000. Predicted molecular weight: 42 kDa.Can be blocked with beta Actin peptide (ab13772). Abcam recommends blocking and incubation using 5% BSA as we have found that use of 5% milk significantly reduces the intensity of the signal obtained. Please refer to WB image for further details. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
-
Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P60706 Chicken
- SwissProt: P60712 Cow
- SwissProt: P60708 Horse
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- SwissProt: P29751 Rabbit
- SwissProt: P60711 Rat
- SwissProt: P60713 Sheep
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
- Unigene: 94978 Rat
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody
- Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin antibody [mAbcam 8226] - Loading Control images
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![Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/images/8/ab8226/beta-Actin-Primary-antibodies-ab8226-27.jpg)
Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Lanes 1 - 3 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml (5% BSA BLOCK)
Lanes 4 - 6 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml (5% MILK BLOCK)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 8 minutes -
![Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/Images/8/ab8226/ab8226_4.jpg)
Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Immunofluorescence using ab8226 at 5ug/ml incubated for 1 hour on Rat Colon Cancer cells.
Cells were fixed with ice-cold methanol for 5 mins, then for all following steps, permeabilised in TBS-T for 30 mins, blocked with 5% BSA for 30 mins and then washed in TBS-T. Secondary antibody was Alexa Fluor 488 goat anti-mouse IgG at 1/1000 incubated for 1 hour. Cells were counterstained with DAPI. Image at 400X magnification. All incubations were at room temperature.
The beta actin fibres can be seen arrayed around the edge of the cells.
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![Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/Images/8/ab8226/ab8226_3.jpg)
Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 0.5 µg/ml + HeLa cell lysate
Secondary
Goat polyclonal to mouse IgG H&L (HRP) at 1/5000 dilution
Performed under non-reducing conditions.
Predicted band size : 42 kDa
Observed band size : 42 kDa
Exposure time : 30 seconds -
![Immunocytochemistry - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/images/8/ab8226/beta-Actin-Primary-antibodies-ab8226-30.jpg)
Immunocytochemistry - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)This image is a courtesy of Anonymous Abreviewab8226 staining beta Actin in human AGS cells by Immunocytochemistry. Cells were fixed with formaldehyde, permeabilized with 0.025% Triton-X in TBS and blocking with 5% serum was performed for 1 hour at 230C. Samples were incubated with primary antibody (1/500) for 1 hour at 23°C. An HRP®-conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/images/8/ab8226/beta-Actin-Primary-antibodies-ab8226-31.jpg)
Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)This image is a courtesy of Anonymous Abreviewab8226 staining beta Actin in human gastric epithelial AGS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde. Permeabilization and blocking was carried out using 5% BSA containing 0.025% Triton X in TBS for 1 hour at 23°C. Primary antibody was used at 5µg/ml for 1 hour at 23°C. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/1000 dilution.
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![Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/images/8/ab8226/beta-Actin-Primary-antibodies-ab8226-97.jpg)
Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Exposure time : 10 seconds -
![Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)](http://a.abcam.com/ps/datasheet/Images/8/ab8226/ab8226_2.jpg)
Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Lane 1 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution
Lane 2 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/10000 dilution
Lanes 3 - 4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/500 dilution
Lane 1 : HeLa Cell lysate
Lane 2 : HeLa Cell lysate
Lane 3 : 293 Cell lysate
Lane 4 : 3T3 Mouse Cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 42 kDa
Exposure time : 10 seconds
References for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)
This product has been referenced in:
- Pham AM et al. Replication in cells of hematopoietic origin is necessary for Dengue virus dissemination. PLoS Pathog 8:e1002465 (2012). IHC-P . Read more (PubMed: 22241991) »
- Didangelos A et al. Novel Role of ADAMTS-5 Protein in Proteoglycan Turnover and Lipoprotein Retention in Atherosclerosis. J Biol Chem 287:19341-5 (2012). WB . Read more (PubMed: 22493487) »
