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Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

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Overview

Product name

Anti-beta Actin antibody [mAbcam 8226] - Loading Control
See all beta Actin products (9) ...

Description

Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control

Specificity

Does not cross-react with adult cardiac, smooth, or skeletal muscle actin.

Tested applications

ICC/IF, ICC, Flow Cyt, IHC-FrFl, IHC-P, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, African Green Monkey, Chinese Hamster, Armenian Hamster

Predicted to work with

Sheep, Horse, Guinea pig

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

Read Abcam's proprietary immunogen policy

(Peptide available as ab13772.)

General notes

The concentration of this antibody is batch dependent.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

mAbcam 8226

Myeloma

Sp2/0-Ag14

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin

  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) image (enlarge)

  • Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) image (enlarge)

  • Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab8226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Involvement in disease

Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.

Sequence similarities

Belongs to the actin family.

Post-translational
modifications

ISGylated.

Cellular localization

Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accessionP60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • cytoplasmic 1 antibody
  • N-terminally processed antibody
  • A X actin like protein antibody
  • ACTB antibody
  • ACTB_HUMAN antibody
  • Actin antibody
  • Actin cytoplasmic 1 antibody
  • Actx antibody
  • Beta cytoskeletal actin antibody
  • Beta-actin antibody
  • Melanoma X actin antibody
  • PS1TP5 binding protein 1 antibody
  • PS1TP5BP1 antibody
see all

Database links

see all

Anti-beta Actin antibody [mAbcam 8226] - Loading Control images:

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Lanes 1 - 3 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml (5% BSA BLOCK)
Lanes 4 - 6 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml (5% MILK BLOCK)

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa


Exposure time : 8 minutes

  Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Immunofluorescence using ab8226 at 5ug/ml incubated for 1 hour on Rat Colon Cancer cells.

Cells were fixed with ice-cold methanol for 5 mins, then for all following steps, permeabilised in TBS-T for 30 mins, blocked with 5% BSA for 30 mins and then washed in TBS-T. Secondary antibody was Alexa Fluor 488 goat anti-mouse IgG at 1/1000 incubated for 1 hour. Cells were counterstained with DAPI. Image at 400X magnification. All incubations were at room temperature.

The beta actin fibres can be seen arrayed around the edge of the cells.

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 0.5 µg/ml + HeLa cell lysate

Secondary
Goat polyclonal to mouse IgG H&L (HRP) at 1/5000 dilution

Performed under non-reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa


Exposure time : 30 seconds

  Immunocytochemistry - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Immunocytochemistry - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

ab8226 staining beta Actin in human AGS cells by Immunocytochemistry. Cells were fixed with formaldehyde, permeabilized with 0.025% Triton-X in TBS and blocking with 5% serum was performed for 1 hour at 230C. Samples were incubated with primary antibody (1/500) for 1 hour at 23°C. An HRP®-conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody.

This image is a courtesy of Anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Immunocytochemistry/ Immunofluorescence - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

ab8226 staining beta Actin in human gastric epithelial AGS cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde. Permeabilization and blocking was carried out using 5% BSA containing 0.025% Triton X in TBS for 1 hour at 23°C. Primary antibody was used at 5µg/ml for 1 hour at 23°C. An Alexa Fluor 488® conjugated goat polyclonal to mouse IgG was used as the secondary antibody at a 1/1000 dilution.

This image is a courtesy of Anonymous Abreview

See Abreview

  Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)


developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 42 kDa


Exposure time : 10 seconds

Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623).
Loading Control (ab8226) used at 1 µg/ml.

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK293 cell lysate
Lane 5 : HepG2 cell lysate

Lysates loaded at 20 µg per lane.

  Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Western blot - beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Lane 1 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/1000 dilution
Lane 2 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/10000 dilution
Lanes 3 - 4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1/500 dilution

Lane 1 : HeLa Cell lysate
Lane 2 : HeLa Cell lysate
Lane 3 : 293 Cell lysate
Lane 4 : 3T3 Mouse Cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728) at 1/5000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa


Exposure time : 10 seconds

References for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

This product has been referenced in:

  • Xu Bet al. Proliferating cell nuclear antigen (PCNA) regulates primordial follicle assembly by promoting apoptosis of oocytes in fetal and neonatal mouse ovaries. PLoS One 6:e16046 (2011). WB; Mouse.Read more (PubMed: 21253613) »
  • Adams JRet al. Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation. Cancer Res 71:2706-17 (2011).Read more (PubMed: 21324922) »

See all 92 publications for this product

Publishing research using ab8226? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"