beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

Product Name 

beta Actin antibody [mAbcam 8226] - Loading Control

Product type 

Primary antibodies

Description 

Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

(Peptide available as ab13772.)

Reacts with 

Hu, Ms, Rat, AGMk, ArHm, Chk, ChHm, Cow, Dog, Pig, Rb

Predicted to react 
(species key)

Gpig, Hrs, Shp
(Due to sequence homology)

Specificity 

Does not cross-react with adult cardiac, smooth, or skeletal muscle actin.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 5 µg/ml.
IP: 1:100
WB: 1/500 - 1/10000. Predicted molecular weight: 42 kDa. Can be blocked with beta Actin peptide (ab13772).

Abcam recommends blocking and incubation using 5% BSA as we have found that use of 5% milk significantly reduces the intensity of the signal obtained. Please refer to WB image for further details.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Isotype/Loading Controls >> Loading Controls >> Beta Actin

Relevance 

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.
Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) "in cells undergoing adipose differentiation actin synthesis decreases by 90%".

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

mAbcam 8226

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

The concentration of this antibody is batch dependent.

See below for beta Actin antibody [mAbcam 8226] - Loading Control images, references, products related to ab8226 and other tools.

beta Actin antibody [mAbcam 8226] - Loading Control images:

References for beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

  This product has been used in: (two most recent references)

Xu K et al. Lunatic Fringe-mediated Notch signaling is required for lung alveogenesis. Am J Physiol Lung Cell Mol Physiol 298:L45-56 (2010). WB; Mouse. PubMed: 19897741

Vreede FT et al. Mechanisms and functional implications of the degradation of host RNA polymerase II in influenza virus infected cells. Virology 396:125-34 (2010). WB; Human. PubMed: 19875144

See all 78 publication references for this product.


If you publish research using ab8226 please let us know so that we can cite the reference on this datasheet.

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