You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product namebeta Actin peptideSee all beta Actin proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 1 - 100 of Human beta Actin.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab8224 - beta Actin antibody [mAbcam 8224] - Loading Control, ab8226 - beta Actin antibody [mAbcam 8226] - Loading Control, ab8227 - beta Actin antibody - Loading Control, ab8229 - beta Actin antibody, ab13822 - beta Actin antibody - Loading Control and ab75186 - beta Actin antibody - Loading Control.
  • Protein length375 amino acids

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab13772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml. This peptide can be used with studies using ab8227.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        A26C1AA26C1BACTB
        ACTB_HUMANActin cytoplasmic 1Actxbeta cytoskeletal actinBeta-actinE430023M04RikMGC128179PS1TP5 binding protein 1PS1TP5BP1
      see all
  • FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similaritiesBelongs to the actin family.
  • Post-translational
    modifications    ISGylated.
  • Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    beta Actin peptide images

    • Western blot - beta Actin antibody - Loading Control (ab8227)
      Western blot - beta Actin antibody - Loading Control (ab8227)
      All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1/1000 dilution

      Lane 1 : HeLa nuclear lysate
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HEK 293 cell lysate
      Lane 6 : HeLa nuclear lysate with beta Actin peptide (ab13772) at 1 µg/ml
      Lane 7 : HeLa whole cell lysate with beta Actin peptide (ab13772) at 1 µg/ml
      Lane 8 : A431 cell lysate with beta Actin peptide (ab13772) at 1 µg/ml
      Lane 9 : Jurkate cell lysate with beta Actin peptide (ab13772) at 1 µg/ml
      Lane 10 : HEK 293 cell lysate with beta Actin peptide (ab13772) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution

      Observed band size : 41.7 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 seconds

    References for beta Actin peptide (ab13772)

    ab13772 has not yet been referenced specifically in any publications.

    Publishing research using ab13772 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab13772 Submit a Question for ab13772

    There are currently no Abreviews or Questions for ab13772.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"