Overview
- Product nameAnti-beta Actin (phospho Y55 + Y53) antibodySee all beta Actin primary antibodies ...
- DescriptionRabbit polyclonal to beta Actin (phospho Y55 + Y53)
- SpecificityThis antibody detects endogenous levels of beta Actin only when phosphorylated at tyrosine 53/55.
- Tested applicationsELISA, IHC-P, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthesized phosphopeptide derived from human beta Actin around the phosphorylation site of tyrosine 53/55.
- Positive controlHuman skeletal muscle tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab59381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/5000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| WB | WB: Use at an assay dependent concentration. |
Target
- FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
- Sequence similaritiesBelongs to the actin family.
- Post-translational
modificationsISGylated. - Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
- Unigene: 328431 Mouse
- Unigene: 391967 Mouse
see all
Target information above from: UniProt accession
P60709
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- A26C1A antibodyA26C1B antibodyACTB antibody
- ACTB_HUMAN antibodyActin cytoplasmic 1 antibodyActx antibodybeta cytoskeletal actin antibodyBeta-actin antibodyE430023M04Rik antibodyMGC128179 antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody
see all
Anti-beta Actin (phospho Y55 + Y53) antibody images
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Immunohistochemistry (Paraffin-embedded sections) - beta Actin (phospho Y55 + Y53) antibody (ab59381)Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using ab59381 at a 1/50 dilution.Left image un-treated.
Right image treated with phosphopeptide.
References for Anti-beta Actin (phospho Y55 + Y53) antibody (ab59381)
ab59381 has not yet been referenced specifically in any publications.
