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Synthetic peptide conjugated to KLH, corresponding to amino acids 33-42 of Human beta Amyloid 1-42.
Do not store antibody diluted below 0.5 mg/ml.
We have received positive and negative feedback from customers for the WB application. WB is not batch-tested for this antibody and we are not able to cover WB under our Abpromise guarantee.
Our Abpromise guarantee covers the use of ab10148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18673368|
|ICC||Use at an assay dependent concentration. PubMed: 22003387|
ab10148 staining beta Amyloid 1-42 in Rat astrocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 5% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/100) for 18 hours at 37°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ab10148 at a 1/50 dilution staining mouse AD brain tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using a biotinylated Goat anti-Rabbit antibody.
This image is courtesy of an Abreview submitted on 15 March 2006.
ab10148 staining mouse skeletal muscle tissue sections by IHC-Fr. Sections were acetone fixed and blocked in 5% BSA for 30 minutes at RT prior to incubating with ab10148, diluted 1/100, for 12 hours at 4°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"