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Synthetic peptide conjugated to KLH, corresponding to amino acids 33-42 of Human beta Amyloid 1-42.
Our Abpromise guarantee covers the use of ab10148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 18673368|
|ICC||Use at an assay dependent concentration. PubMed: 22003387|
|WB||Use at an assay dependent concentration. PubMed: 19151372|
ab10148 staining beta Amyloid 1-42 in Rat astrocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 5% serum for 1 hour at 4°C. Samples were incubated with primary antibody (1/100) for 18 hours at 37°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ab10148 at a 1/50 dilution staining mouse AD brain tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using a biotinylated Goat anti-Rabbit antibody.
This image is courtesy of an Abreview submitted on 15 March 2006.
ab10148 staining mouse skeletal muscle tissue sections by IHC-Fr. Sections were acetone fixed and blocked in 5% BSA for 30 minutes at RT prior to incubating with ab10148, diluted 1/100, for 12 hours at 4°C. A FITC conjugated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
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