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Product name
Anti-beta Bax antibody
Description
Sheep polyclonal to beta Bax
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: LTTAPAPPSLPPATPLGPWAFWSRSQWC, corresponding to amino acids 172-199 of Human beta Bax.
LTTAPAPPSLPPATPLGPWAFWSRSQWC
Positive control
in vitro translated Bax beta
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is provided as a 0.2 µm sterile filtered solution.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cell Biology >> Apoptosis >> Mitochondrial
Cell Biology >> Apoptosis >> Intracellular >> Bcl2 Family
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Immunoprecipitation - beta Bax antibody (ab16143)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab16143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IP
Application notes
IP: Use at an assay dependent dilution.
Is unsuitable for WB. Will not detect denatured protein.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
beta Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the Bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse Bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the beta Bax protein before a stop codon is encountered during translation. The beta Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain. beta Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with alpha Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. beta Bax mRNA was very abundant in brain and duodenum with almost no alpha Bax mRNA detected in these tissues.
Cellular localization
Cytoplasmic
Alternative names
- Apoptosis regulator Bax cytoplasmic isoform beta antibody
- BAX antibody
- Bax B antibody
see all
Database links
- Entrez Gene: 581 Human
- Omim: 600040 Human
- SwissProt: Q07814 Human
Anti-beta Bax antibody images:
Immunoprecipitation - beta Bax antibody (ab16143)
Immunoprecipitation of in vitro translated Bax alpha (1) and Bax beta (2) using Bax antibody clone 6A7, Bax alpha native (3) and denatured protein(4) using Bax beta antibody ab16143, and Bax beta native (5) and denatured (6) protein using Bax beta antibody ab16143. Ab16143 immunoprecipitated Bax beta strongly however did not detect Bax beta by western blotting.
References for Anti-beta Bax antibody (ab16143)
ab16143 has not yet been referenced specifically in any publications.
Publishing research using ab16143? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"