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Products:Signal Transduction >> Protein Trafficking >> Chaperones
Overview
Product name
Anti-beta COP antibody [M3A5]
See all beta COP products (4) ...
Description
Mouse monoclonal [M3A5] to beta COP
Specificity
Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
Tested applications
WB, IP, IHC-FoFr, ICC, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Chicken, Hamster, Cow, Dog, Human
Immunogen
Full length native protein (purified). (Goose)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Ascites fluid
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
May be used for studies on the effects of various agents influencing energy status, on disrupting the Golgi complex, or on altering the activity of G-proteins or small GTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinity purification of the beta-COP protein.
Clonality
Monoclonal
Clone number
M3A5
Isotype
IgG1
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Chaperones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
IHC-FoFr
ICC
ICC/IF
Application notes
IHC-F: Use at an assay dependant dilution.
ICC: Use at an assay dependant dilution.
ICC/IF: 1/20 - 1/50. (CHO cells)
IP: Use at an assay dependant dilution.
WB: 1/50 - 1/1000. (stacked Golgi membranes from rat liver extract)
Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.
Cellular localization
The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Alternative names
- Beta-coat protein antibody
- betacop antibody
- Coatomer beta subunit antibody
see all
Database links
- Entrez Gene: 423063 Chicken
- Entrez Gene: 1315 Human
- Omim: 600959 Human
- SwissProt: P53618 Human
- SwissProt: Q6GTT7 Human
- SwissProt: P23514 Rat
- Unigene: 339278 Human
References for Anti-beta COP antibody [M3A5] (ab6324)
This product has been referenced in:
- Ginger RSet al. SLC24A5 Encodes a trans-Golgi Network Protein with Potassium-dependent Sodium-Calcium Exchange Activity That Regulates Human Epidermal Melanogenesis. J Biol Chem 283:5486-95 (2008). ICC/IF; Human.Read more (PubMed: 18166528) »
- Matsushima Set al. Involvement of multiple efflux transporters in hepatic disposition of fexofenadine. Mol Pharmacol 73:1474-83 (2008).Read more (PubMed: 18245269) »
See all 5 publications for this product
Publishing research using ab6324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"