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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab24359? Please let us know so that we can cite the reference in this datasheet
ab24359 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - beta COP antibody (ab24359)
All lanes : Anti-beta COP antibody (ab24359) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 110 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab24359 detected a band of ~ 100 kDa in mouse and rat brain lysate. It also detected a band of ~ 100 kDa in NIH 3T3 cells and rat liver cells (data not shown). ab24359 is predicted to react with human beta cop due to 100% identity with the immunogen, however in our hands the antibody failed to detect a band in human brain lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta COP antibody (ab24359)
Image courtesy of Human Protein Atlas
ab24359 staining beta COP in paraffin embedded Human pancreas. The tissue was incubated with ab24359 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Further results for this antibody can be found at www.proteinatlas.org
Immunocytochemistry/ Immunofluorescence - beta COP antibody (ab24359)
ICC/IF image of ab24359 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab24359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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