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Products:Signal Transduction >> Protein Trafficking >> Chaperones
Overview
Product name
Anti-beta COP antibody
See all beta COP products (4) ...
Description
Rabbit polyclonal to beta COP
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Rat beta COP.
Positive control
Rat and mouse brain lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Chaperones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
IHC-P: 1/400Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml
Target
Relevance
The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.
Cellular localization
The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Alternative names
- Beta-coat protein antibody
- betacop antibody
- Coatomer beta subunit antibody
see all
Database links
- Entrez Gene: 423063 Chicken
- Entrez Gene: 1315 Human
- Omim: 600959 Human
- SwissProt: P53618 Human
- SwissProt: Q6GTT7 Human
- SwissProt: Q9JIF7 Mouse
- SwissProt: P23514 Rat
- Unigene: 339278 Human
Anti-beta COP antibody images:
Western blot - beta COP antibody (ab24359)
All lanes : Anti-beta COP antibody (ab24359) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 110 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab24359 detected a band of ~ 100 kDa in mouse and rat brain lysate. It also detected a band of ~ 100 kDa in NIH 3T3 cells and rat liver cells (data not shown). ab24359 is predicted to react with human beta cop due to 100% identity with the immunogen, however in our hands the antibody failed to detect a band in human brain lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - beta COP antibody (ab24359)
Image courtesy of Human Protein Atlas
ab24359 staining beta COP in paraffin embedded Human pancreas. The tissue was incubated with ab24359 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
Further results for this antibody can be found at www.proteinatlas.org
Immunocytochemistry/ Immunofluorescence - beta COP antibody (ab24359)
ICC/IF image of ab24359 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab24359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-beta COP antibody (ab24359)
ab24359 has not yet been referenced specifically in any publications.
Publishing research using ab24359? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"