beta COP antibody (ab2899)

Product Name 

beta COP antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to beta COP

Immunogen 

Synthetic peptide: E(496)AGEL KPEEEITVGP VQK(513).< Immunizing peptide corresponds to amino acid residues 496 - 513 of rat beta-COP.
This sequence is completely conserved in human beta-COP.
BLAST 'EAGELKPEEEITVGPVQK' with or

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Immunofluorescence: 1/2000.
IP: Use at an assay dependent dilution (PMID 18556652).
Western blot: use at duilution of 1/1000.

Optimal dilutions / concentrations are best determined by the end user.

By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate.

Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.

Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

Cellular localization 

The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins

Relevance 

The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

PBS, 1 mg/ml BSA, 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

4.400 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for beta COP antibody images, references, products related to ab2899 and other tools.

beta COP antibody images:

References for beta COP antibody (ab2899)

  This product has been used in: (two most recent references)

Juanes ME et al. Biosynthesis of prion protein nucleocytoplasmic isoforms by alternative initiation of translation. J Biol Chem 284:2787-94 (2009). PubMed: 19059915

Saito K et al. TANGO1 facilitates cargo loading at endoplasmic reticulum exit sites. Cell 136:891-902 (2009). ICC/IF; Human. PubMed: 19269366

See all 8 publication references for this product.


If you publish research using ab2899 please let us know so that we can cite the reference on this datasheet.

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