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Products:Signal Transduction >> Protein Trafficking >> Chaperones
Overview
Product name
Anti-beta COP antibody
See all beta COP products (4) ...
Description
Rabbit polyclonal to beta COP
Tested applications
WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: E(496)AGELKPEEEITVGPVQK(513). Immunizing peptide corresponds to amino acid residues 496 - 513 of rat beta-COP. This sequence is completely conserved in human beta-COP.
EAGELKPEEE ITVGPVQK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Chaperones
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 110 kDa.
ICC/IF: 1/2000(Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.)
IP: Use at an assay dependent dilution. (PubMed: 18556652)
Target
Relevance
The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.
Cellular localization
The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Alternative names
- Beta-coat protein antibody
- betacop antibody
- Coatomer beta subunit antibody
see all
Database links
- Entrez Gene: 1315 Human
- Omim: 600959 Human
- SwissProt: P53618 Human
- SwissProt: Q6GTT7 Human
- SwissProt: Q9JIF7 Mouse
- SwissProt: P23514 Rat
- Unigene: 339278 Human
Anti-beta COP antibody images:
Western blot - beta COP antibody (ab2899)
Western blot of beta-COP on rat brain homogenate using ab2899.
Immunocytochemistry/ Immunofluorescence - beta COP antibody (ab2899)
ab2899 at 1/1000 staining human HeLa cells by ICC/IF. The cells were paraformaldeyhde fixed and blocked with fish gelatine prior to incubation with the antibody for 1 hour. An Alexa Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-beta COP antibody (ab2899)
This product has been referenced in:
- Johnson JSet al. AAV Exploits Subcellular Stress Associated with Inflammation, Endoplasmic Reticulum Expansion, and Misfolded Proteins in Models of Cystic Fibrosis. PLoS Pathog 7:e1002053 (2011).Read more (PubMed: 21625534) »
- Carvou Net al. Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum. J Cell Sci 123:1262-73 (2010). ICC/IF; Human.Read more (PubMed: 20332109) »
See all 12 publications for this product
Publishing research using ab2899? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"