beta COP antibody [maD] (ab6323)

Product Name 

beta COP antibody [maD]

Product type 

Primary antibodies

Description 

Mouse monoclonal [maD] to beta COP

Immunogen 

Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COP (beta).

Reacts with 
(species key)

Hu, Rat, Hm, Mk

Specificity 

Recognises an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
AF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
ICC: Use at an assay dependant dilution.
ICC/IF: 1/80 using methanol/acetone or paraformaldehyde fixation, followed by a permeabilization step. Strong immunofluorescent staining is confined mainly to the central perinuclear area in the cell.
WB: 1/1000.
May be used for micro-injection into cells.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins

Relevance 

The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

maD

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Ascites fluid

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

4.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for beta COP antibody [maD] images, references, products related to ab6323 and other tools.

beta COP antibody [maD] images:

References for beta COP antibody [maD] (ab6323)

  This product has been used in: (two most recent references)

Burman JL et al. Scyl1 regulates Golgi morphology. PLoS One 5:e9537 (2010). WB. PubMed: 20209057

Blagoveshchenskaya A et al. Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. J Cell Biol 180:803-12 (2008). WB. PubMed: 18299350

See all 6 publication references for this product.


If you publish research using ab6323 please let us know so that we can cite the reference on this datasheet.

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