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Anti-beta COP antibody [maD] (ab6323)

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Overview

Product name

Anti-beta COP antibody [maD]
See all beta COP products (4) ...

Description

Mouse monoclonal [maD] to beta COP

Specificity

Recognises an epitope in the beta-COP protein (110kD). The antibody stains the periphery of the Golgi complex in immunohistology using cultured cells.

Tested applications

IHC-Fr, ICC/IF, Functional Studies, WB, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Hamster, Human, Monkey

Immunogen

Synthetic peptide conjugated to KLH, corresponding to amino acids 701-715 of COP (beta).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites fluid

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

maD

Isotype

IgG1

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Signal Transduction >> Protein Trafficking >> Chaperones

  • Western blot - beta COP antibody [maD] (ab6323)Western blot - beta COP antibody [maD] (ab6323) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - beta COP antibody [maD] (ab6323)Immunocytochemistry/ Immunofluorescence - beta COP antibody [maD] (ab6323) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The Golgi complex is a key organelle where processing and sorting of newly synthesized proteins occurs. Membrane traffic from the endoplasmic reticulum (ER) to the Golgi complex and from the Golgi complex to the different final cellular destinations is believed to be mediated by carrier vesicles. Two populations of coated vesicles mediate biosynthetic membrane traffic between the different membrane-bound compartments. Clathrin-coated vesicles carry proteins to endocytic organelles and secretory granules, whilst non-clathrin-coated vesicles are involved in intra-Golgi transport and transport from the ER to the Golgi complex. Beta COP is a member of a set of protein which are believed to associate with the non-clathrin coated vesicles. Golgi-derived non-clathrin-coated vesicles are believed to act as bulk carriers, whereas clathrin-coated vesicles carry a selective cargo of membrane proteins.

Cellular localization

The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.

Alternative names

  • Beta-coat protein antibody
  • betacop antibody
  • Coatomer beta subunit antibody
  • Coatomer protein complex subunit beta 1 antibody
  • Coatomer protein complex subunit beta antibody
  • Coatomer subunit beta antibody
  • COPB antibody
  • COPB1 antibody
  • DKFZp761K102 antibody
  • FLJ10341 antibody
see all

Database links

Anti-beta COP antibody [maD] images:

  Western blot - beta COP antibody [maD] (ab6323)

Western blot - beta COP antibody [maD] (ab6323)

ab6323 at a 1/1000 dilution staining ~ 105 kDa beta COP in melanoma cell lysate by Western blot

  Immunocytochemistry/ Immunofluorescence - beta COP antibody [maD] (ab6323)

Immunocytochemistry/ Immunofluorescence - beta COP antibody [maD] (ab6323)

Untreated HEK293 cells incubated with the COPB ab6323 in a dilution of 1/100. This picture was kindly submitted as part of the review by Evelien Gellynck.

References for Anti-beta COP antibody [maD] (ab6323)

This product has been referenced in:

  • Burman JLet al. Scyl1 regulates Golgi morphology. PLoS One 5:e9537 (2010). WB.Read more (PubMed: 20209057) »
  • Sharma Pet al. Endoplasmic reticulum protein targeting of phospholamban: a common role for an N-terminal di-arginine motif in ER retention? PLoS One 5:e11496 (2010). WB, ICC/IF; Human.Read more (PubMed: 20634894) »

See all 7 publications for this product

Publishing research using ab6323? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"