Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin [E247] antibody

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample Mouse Cell (mouse Embryonic stem cell)
Specification mouse Embryonic stem cell
Permeabilization Yes - 0.1% triton X
Fixative Methanol:acetone

Other product details

Incubation time 2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: block buffer
Dilution 1/1000

Secondary antibody

Dilution 1/200

Additional data

Additional Notes Staining on WT cells shown (green is beta-catenin, blue is DAPI). I also stained a cell line known to have reduced beta-catenin expression and saw correspondingly reduced expression with this antibody (not shown).

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Submitted Jul 08 2014