Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody [E247]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
aglandular stomach, epithelium
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methacarn

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris-NaCl-Tween buffer
Dilution
1/2000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Dako Polyclonal Swine Anti - Rabbit Immunoglobuli
Host species: Pig
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Propidium iodide counterstaining (nuclei) and TSA labeling kit
Username

F Hernandez-Blazquez

Verified customer

Submitted Mar 17 2016

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