Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody [E247]

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification aglandular stomach, epithelium
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Methacarn

Other product details

Incubation time 14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris-NaCl-Tween buffer
Dilution 1/2000

Secondary antibody

Dilution 1/400
Name Non-Abcam antibody was used: Dako Polyclonal Swine Anti - Rabbit Immunoglobuli
Host species: Pig
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes Propidium iodide counterstaining (nuclei) and TSA labeling kit

F Hernandez-Blazquez

Verified customer

Submitted Mar 17 2016